Evidence map›Paper›PMID 38058747›Full record

ArticleJVS-vascular science2023

Different methods of bone marrow harvesting influence cell characteristics and purity, affecting clinical outcomes.

Eugenio Caradonna, Elisabetta Mormone, Enrico Maria Centritto, Andrea Mazzanti, Stefano Papini, Mara Fanelli, Lella Petrella, Arnolfo Petruzziello, Michele Angelo Farina, Eleonora Farina and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in JVS-vascular science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Trial
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 6 institutions in 1 country.

Eugenio CaradonnaGemelli Molise S.p.A., Campobasso, Italy.
Elisabetta MormoneInstitute for Stem-Cell Biology, Regenerative Medicine and Innovative Therapies, Fondazione IRCCS Casa Sollievo della Sofferenza, Foggia, Italy.
Enrico Maria CentrittoGemelli Molise S.p.A., Campobasso, Italy.
Andrea MazzantiDepartment of Molecular Medicine, University of Pavia, Pavia, Italy.
Stefano PapiniClinical and Research Laboratory, Gemelli Molise S.p.A., Campobasso, Italy.
Mara FanelliLaboratorio di Diagnostica Molecolare, Gemelli Molise S.p.A., Campobasso, Italy.
Lella PetrellaLaboratorio di Diagnostica Molecolare, Gemelli Molise S.p.A., Campobasso, Italy.
Arnolfo PetruzzielloUOC Patologia Clinica, Dipartimento dei Servizi Sanitari, AORN CASERTA, Caserta, Italy.
Michele Angelo FarinaStudio Medico Farina, Caserta, Italy.
Eleonora FarinaStudio Medico Farina, Caserta, Italy.
Bruno AmatoDepartment of Public Health, University of Naples Federico II, Naples, Italy.
Carlo Maria De FilippoCardiovascular Department, Gemelli Molise S.p.A, Campobasso, Italy.
Emilio VanoliDepartment of Molecular Medicine, University of Pavia, Pavia, Italy.
University of Molise · ITRina Services (Italy) · ITUniversity of Pavia · ITCasa Sollievo della Sofferenza · ITServizio Sanitario Nazionale · ITUniversity of Naples Federico II · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Bone marrow (BM)-derived stem cells were implanted to induce angiogenesis in patients with no-option critical limb-threatening ischemia. Considering the potential for this therapy, conflicting results related to BM harvesting methods have been reported that could affect stem cell concentrations and quality. Methods: A total of 75 patients with no-option critical limb-threatening ischemia were treated with BM implantation. For 58 patients, BM was harvested using a BM aspirate concentrate system (Harvest Technologies; group HT) with a standard aspiration needle, followed by an automated centrifugation process, to produce BM aspirate concentrate. For 17 patients, BM was harvested using the Marrow Cellution system (Aspire Medical Innovation; group MC). CD34 Results: The retrospective analysis of a subset group after adjustment for age shows that the quality of BM obtained using the Marrow Cellution system is better, in terms of purity, than the classic harvesting method before centrifugation. Harvested BM before centrifugation is characterized by a higher percentage of CD133 Conclusions: BM manipulation, such as centrifugation, affects the quality and number of stem cells, with detrimental consequences on clinical outcomes, as reflected by the different amputation rates between the two groups.

Indexed as

AngiogenesisBone marrowHematopoietic stem cellsPeripheral arterial diseaseVery small embryonic-like stem cells

Identifiers

PMID38058747
PMCPMC10696233
OpenAlexW4387642786

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.