Evidence map›Paper›PMID 38058301›Full record

ArticleiScience2023

ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1.

Ozlem Calbay, Ravi Padia, Mahmuda Akter, Lei Sun, Bin Li, Nicole Qian, Jianhui Guo, Zheng Fu, Lingtao Jin, Shuang Huang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Ozlem CalbayDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Ravi PadiaDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Mahmuda AkterDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Lei SunDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Bin LiDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Nicole QianDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Jianhui GuoDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
Zheng FuDepartment of Human and Molecular Genetics, VCU Institute of Molecular Medicine, VCU Massey Cancer Center, Virginia Commonwealth University, School of Medicine, Richmond, VA 23298, USA.
Lingtao JinDepartment of Molecular Medicine, University of Texas Health San Antonio, San Antonio, TX 78229, USA.
Shuang HuangDepartment of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville, FL 32610, USA.
University of Florida · USThe University of Texas Health Science Center at San Antonio · USVirginia Commonwealth University · US

Funding

Novel protein kinase signaling associated with platinum resistance in ovarian cancerR01CA256482 · NCI · UNIVERSITY OF FLORIDA · PI HUANG, SHUANG, SONG, LINA · 2021 to 2025
$2.2M
Impact of microRNA processing on EMT of ovarian cancer cellsR01CA222467 · NCI · UNIVERSITY OF FLORIDA · PI HUANG, SHUANG · 2018 to 2022
$1.7M
NCI NIH HHS R01 CA222467NCI NIH HHS R01 CA256482
6 · The paper itself

Abstract

Canonical pyroptosis is type of programmed cell death depending on active caspase-1, and the inflammasome carries out caspase-1 activation. Here, we showed that docosahexaenoic acid (DHA) induced ovarian cancer cell deaths in caspase-1-dependent manner. DHA increased caspase-1 activity and led to interleukin-1β secretion and gasdermin D cleavage while disulfiram inhibited DHA-induced cell death, suggesting that DHA triggered pyroptosis. Intriguingly, ASC, the molecule recruiting caspase-1 to inflammasome for activation, was dispensable for DHA-induced pyroptosis. Instead, we observed remarkable elevation in caspase-1 abundance concurrent with the activation of caspase-1 in DHA-treated cells. As ectopically overexpressing caspase-1 resulted in robust amount of active caspase-1, we reason that DHA activates caspase-1 and pyroptosis through the generation of excessive amount of caspase-1 protein. Mechanistically, DHA increased caspase-1 by specifically accelerating caspase-1 protein synthesis via the p38

Indexed as

CancerImmunologyMolecular biology

Identifiers

PMID38058301
PMCPMC10696124
OpenAlexW4388461935

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.