Evidence map›Paper›PMID 38053291›Full record

ArticleBMB reports2024

Genome-wide in-locus epitope tagging of Arabidopsis proteins using prime editors.

Cheljong Hong, Jun Hee Han, Gue-Ho Hwang, Sangsu Bae, Pil Joon Seo

Open access · greenAbstract readNews
PubMed Full text at PMC
In one paragraph

Article in BMB reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 0 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Cheljong HongDepartment of Chemistry, Seoul National University, Seoul 08826, Korea.
Jun Hee HanDepartment of Chemistry, Hanyang University, Seoul 04673, Korea.
Gue-Ho HwangMedical Research Center of Genomic Medicine Institute, Seoul National University College of Medicine, Seoul 03080, Korea.
Sangsu BaeMedical Research Center of Genomic Medicine Institute, Seoul National University College of Medicine, Seoul 03080; Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080; Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea.
Pil Joon SeoDepartment of Chemistry, Seoul National University, Seoul 08826; Plant Genomics and Breeding Institute, Seoul National University, Seoul 08826, Korea.
Hanyang University · KRSeoul National University · KRSeoul National University Hospital · KR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Prime editors (PEs), which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusion proteins programmed with prime editing guide RNAs (pegRNAs), can not only edit bases but also install transversions, insertions, or deletions without both donor DNA and double-strand breaks at the target DNA. As the demand for in-locus tagging is increasing, to reflect gene expression dynamics influenced by endogenous genomic contexts, we demonstrated that PEs can be used to introduce the hemagglutinin (HA) epitope tag to a target gene locus, enabling molecular and biochemical studies using in-locus tagged plants. To promote genome-wide in-locus tagging, we also implemented a publicly available database that designs pegRNAs for in-locus tagging of all the Arabidopsis genes. [BMB Reports 2024; 57(1): 66-70].

Indexed as

Arabidopsis ProteinsCRISPR-Cas SystemsDNAGene EditingRNA, Guide, CRISPR-Cas SystemsArabidopsis ProteinsDNARNA, Guide, CRISPR-Cas Systems

Identifiers

PMID38053291
PMCPMC10828436
OpenAlexW4389397579

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.