Evidence map›Paper›PMID 38053140›Full record

ArticleBMC veterinary research2023

The establishment of a recombinase polymerase amplification technique for the detection of mouse poxvirus.

Yuexiao Lian, Mengdi Zhang, Yujun Zhu, Miaoli Wu, Bihong Huang, Li Xiao, Kehang Shi, Peide Li, Feng Cong, Huanan Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC veterinary research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Yuexiao Lian *Guangdong laboratory animals monitoring instituteand Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510663, China.
Mengdi Zhang *Guangdong laboratory animals monitoring instituteand Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510663, China.
Yujun ZhuGuangdong laboratory animals monitoring instituteand Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510663, China.
Miaoli WuGuangdong laboratory animals monitoring instituteand Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510663, China.
Bihong HuangGuangdong laboratory animals monitoring instituteand Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510663, China.
Li XiaoGuangdong laboratory animals monitoring instituteand Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510663, China.
Kehang ShiDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Peide LiWenzhou Engineering Research Center of Pet, Department of Animal Science, Wenzhou Vocational College of Science & Technology, Wenzhou, 325006, China. su-2737@163.com.
Feng CongGuangdong laboratory animals monitoring instituteand Guangdong Provincial Key Laboratory of Laboratory Animals, Guangzhou, 510663, China. 397020703@qq.com.
Huanan WangDepartment of Veterinary Medicine, College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China. hnwang@zju.edu.cn.
Guangdong Laboratory Animals Monitoring Institute · CNZhejiang University · CNWenzhou Vocational College of Science and Technology · CN

Funding

the Fundamental Research Funds for the Central Universities 2022-KYY-517101-0005the Key Research and Development Program of Zhejiang Province 2021C02060the open research fund of Wenzhou Engineering Research Center of Pet WP05the Young Scientist Project of the National key Research and Development Program 2021YFF0703300
6 · The paper itself

Abstract

backgroundEctromelia virus (ECTV) is the causative agent of mousepox in mice. In the past century, ECTV was a serious threat to laboratory mouse colonies worldwide. Recombinase polymerase amplification (RPA), which is widely used in virus detection, is an isothermal amplification method.

resultsIn this study, a probe-based RPA detection method was established for rapid and sensitive detection of ECTV.Primers were designed for the highly conserved region of the crmD gene, the main core protein of recessive poxvirus, and standard plasmids were constructed. The lowest detection limit of the ECTV RT- RPA assay was 100 copies of DNA mol-ecules per reaction. In addition, the method showed high specificity and did not cross-react with other common mouse viruses.Therefore, the practicability of the RPA method in the field was confirmed by the detection of 135 clinical samples. The real-time RPA assay was very similar to the ECTV real-time PCR assay, with 100% agreement.

conclusionsIn conclusion, this RPA assay offers a novel alternative for the simple, sensitive, and specific identification of ECTV, especially in low-resource settings.

Indexed as

Ectromelia virusRecombinasesAnimalsMiceNucleic Acid Amplification TechniquesReal-Time Polymerase Chain ReactionSensitivity and SpecificityRecombinasesCrmD geneDetectionEctromelia virusQualitative analysisRecombinase polymerase amplification

Identifiers

PMID38053140
PMCPMC10698935
OpenAlexW4389356426

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.