Evidence map›Paper›PMID 38052755›Full record

ArticleMikrochimica acta2023

Experimental design for the development of a multiplex antigen lateral flow immunoassay detecting the Southern African Territory (SAT) serotypes of foot-and-mouth disease virus.

Simone Cavalera, Eugenio Alladio, Efrem Alessandro Foglia, Santina Grazioli, Barbara Colitti, Sergio Rosati, Chiara Nogarol, Fabio Di Nardo, Thea Serra, Valentina Testa and 4 more

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Article in Mikrochimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Simone CavaleraDepartment of Chemistry, University of Turin, Via P. Giuria 5, Turin, TO, Italy. simone.cavalera@unito.it.ORCID http://orcid.org/0000-0002-5353-647X
Eugenio AlladioDepartment of Chemistry, University of Turin, Via P. Giuria 5, Turin, TO, Italy.
Efrem Alessandro FogliaNational/OIE/FAO, Reference Centre for FMD and SVD, Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia-Romagna, Via A. Bianchi 9, Brescia, BS, Italy.
Santina GrazioliNational/OIE/FAO, Reference Centre for FMD and SVD, Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia-Romagna, Via A. Bianchi 9, Brescia, BS, Italy.
Barbara ColittiDepartment of Veterinary Science, University of Turin, Largo P. Braccini 5, Grugliasco, TO, Italy.
Sergio RosatiDepartment of Veterinary Science, University of Turin, Largo P. Braccini 5, Grugliasco, TO, Italy.
Chiara NogarolIn3diagnostic s.r.l., Largo P. Braccini, 2, Grugliasco, TO, Italy.
Fabio Di NardoDepartment of Chemistry, University of Turin, Via P. Giuria 5, Turin, TO, Italy.
Thea SerraDepartment of Chemistry, University of Turin, Via P. Giuria 5, Turin, TO, Italy.
Valentina TestaDepartment of Chemistry, University of Turin, Via P. Giuria 5, Turin, TO, Italy.
Claudio BaggianiDepartment of Chemistry, University of Turin, Via P. Giuria 5, Turin, TO, Italy.
Giampietro MaccabianiNational/OIE/FAO, Reference Centre for FMD and SVD, Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia-Romagna, Via A. Bianchi 9, Brescia, BS, Italy.
Emiliana BrocchiNational/OIE/FAO, Reference Centre for FMD and SVD, Istituto Zooprofilattico Sperimentale Della Lombardia E Dell'Emilia-Romagna, Via A. Bianchi 9, Brescia, BS, Italy.
Laura AnfossiDepartment of Chemistry, University of Turin, Via P. Giuria 5, Turin, TO, Italy.

Funding

EuFMD Fund for Applied Research EuFMD-FAR - 2022Italian National Grant for the Research PRC2020_002Project CH4.0 under the MUR program "Dipartimenti di Eccellenza 2023-2027" CUP:D13C22003520001
6 · The paper itself

Abstract

Antigenic lateral flow immunoassays (LFIAs) rely on the non-competitive sandwich format, including a detection (labelled) antibody and a capture antibody immobilised onto the analytical membrane. When the same antibody is used for the capture and the detection (single epitope immunoassay), the saturation of analyte epitopes by the probe compromises the capture and lowers the sensitivity. Hence, several factors, including the amount of the probe, the antibody-to-label ratio, and the contact time between the probe and the analyte before reaching the capture antibody, must be adjusted. We explored different designs of experiments (full-factorial, optimal, sub-optimal models) to optimise a multiplex sandwich-type LFIA for the diagnosis and serotyping of two Southern African Territory (SAT) serotypes of the foot-and-mouth disease virus, and to evaluate the reduction of the number of experiments in the development. Both assays employed single epitope sandwich, so most influencing variables on the sensitivity were studied and individuated. We upgraded a previous device increasing the sensitivity by a factor of two and reached the visual limit of detection of 10

Indexed as

Foot-and-Mouth Disease VirusAnimalsAntibodiesAntigensEpitopesImmunoassayResearch DesignSerogroupAntibodiesAntigensEpitopesAntigen saturationGold nanoparticlesHook effectSandwich test

Identifiers

PMID38052755

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.