Evidence map›Paper›PMID 38051050›Full record

ArticleMicrobiology spectrum2024

Evidence of the simultaneous replications of active viruses in specimens positive for multiple respiratory viruses.

Miyuki Kawase, Reiko Suwa, Satoko Sugimoto, Masatoshi Kakizaki, Yohei Kume, Mina Chishiki, Takashi Ono, Hisao Okabe, Sakurako Norito, Makoto Ujike and 3 more

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 9 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Miyuki KawaseDepartment of Virology III, National Institute of Infectious Disease , Gakuen, Musashimurayama, Tokyo, Japan.
Reiko SuwaDepartment of Virology III, National Institute of Infectious Disease , Gakuen, Musashimurayama, Tokyo, Japan.
Satoko SugimotoDepartment of Virology III, National Institute of Infectious Disease , Gakuen, Musashimurayama, Tokyo, Japan.
Masatoshi KakizakiDepartment of Virology III, National Institute of Infectious Disease , Gakuen, Musashimurayama, Tokyo, Japan.
Yohei KumeDepartment of Pediatrics, School of Medicine, Fukushima Medical University , Hikarigaoka, Fukushima, Japan.
Mina ChishikiDepartment of Pediatrics, School of Medicine, Fukushima Medical University , Hikarigaoka, Fukushima, Japan.
Takashi OnoDepartment of Pediatrics, School of Medicine, Fukushima Medical University , Hikarigaoka, Fukushima, Japan.
Hisao OkabeDepartment of Pediatrics, School of Medicine, Fukushima Medical University , Hikarigaoka, Fukushima, Japan.
Sakurako NoritoDepartment of Pediatrics, School of Medicine, Fukushima Medical University , Hikarigaoka, Fukushima, Japan.
Makoto UjikeFaculty of Veterinary Medicine, Nippon Veterinary and Life Science University , Musashino, Tokyo, Japan.ORCID 0000-0002-7145-7126
Mitsuaki HosoyaDepartment of Pediatrics, School of Medicine, Fukushima Medical University , Hikarigaoka, Fukushima, Japan.
Koichi HashimotoDepartment of Pediatrics, School of Medicine, Fukushima Medical University , Hikarigaoka, Fukushima, Japan.ORCID 0000-0003-4955-6221
Kazuya ShiratoDepartment of Virology III, National Institute of Infectious Disease , Gakuen, Musashimurayama, Tokyo, Japan.ORCID 0000-0002-3375-9799
Fukushima Medical University · JPNational Institute of Infectious Diseases · JPNippon Veterinary and Life Science University · JP

Funding

Japan Agency for Medical Research and Development (AMED) 22fk0108119j0603 and 23fk0108661j0001Japan Agency for Medical Research and Development (AMED) 22kf0108117j0103 and 22fk0108543j0201MEXT | Japan Society for the Promotion of Science (JSPS) 20K06441
6 · The paper itself

Abstract

importanceSince the pandemic of coronavirus diseases 2019, the use of real-time PCR assay has become widespread among people who were not familiar with it in virus detection. As a result, whether a high real-time PCR value in one time test indicates virus transmissibly became a complicated social problem, regardless of the difference in assays and/or amplification conditions, the time and number of diagnostic test during the time course of infection. In addition, the multiple positives in the test of respiratory viruses further add to the confusion in the interpretation of the infection. To address this issue, we performed virus isolation using pediatric SARI (severe acute respiratory infections) specimens on air-liquid interface culture of human bronchial/tracheal epithelial cell culture. The result of this study can be a strong evidence that the specimens showing positivity for multiple agents in real-time PCR tests possibly contain infectious viruses.

Indexed as

PneumoniaRespiratory Tract InfectionsVirus DiseasesVirusesChildHumansReal-Time Polymerase Chain Reactionco-cultivationHBTEC-ALI culturereal-time RT-PCRrespiratory virus

Identifiers

PMID38051050
PMCPMC10783086
OpenAlexW4389348453

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.