Evidence map›Paper›PMID 38049817›Full record

ArticleLipids in health and disease2023

Rotundic acid improves nonalcoholic steatohepatitis in mice by regulating glycolysis and the TLR4/AP1 signaling pathway.

Xing-Yang Shi, Xiao-Min Zheng, Hui-Jie Liu, Xue Han, Lei Zhang, Bei Hu, Shan Li

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lipids in health and disease, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.9field-weighted citation impact, top 13% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Review
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  4. Iranian journal of basic medical sciences · 2026
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Xing-Yang Shi *MOE International Joint Laboratory for Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, P. R. China.
Xiao-Min Zheng *MOE International Joint Laboratory for Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, P. R. China.
Hui-Jie LiuMOE International Joint Laboratory for Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, P. R. China.
Xue HanMOE International Joint Laboratory for Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, P. R. China.
Lei ZhangMOE International Joint Laboratory for Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, P. R. China.
Bei HuDepartment of Emergency Medicine, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, 510030, PR China. hubei@gdph.org.cn.
Shan LiMOE International Joint Laboratory for Synthetic Biology and Medicines, School of Biology and Biological Engineering, South China University of Technology, Guangzhou, 510006, P. R. China. lishan@scut.edu.cn.
South China University of Technology · CNGuangdong Institute for Drug Control · CNSouthern Medical University · CN

Funding

Basic and Applied Basic Research Foundation of Guangdong Province 2021B1515120037Guangdong Provincial Medical Products Administration 2023YDZ02Guangdong Provincial Medical Products Administration 2023ZDZ02High-level Hospital Construction Project of Guangdong Provincial People's Hospital KJ0122019448National Key R&D Projects 2022YFC0870100National Key R&D Projects 2023YFC3040700National Natural Science Foundation of China 82072139Natural Science Foundation of Guangdong Province for Distinguished Young Scholars KJ012021184Special Project for Research and Development in Key areas of Guangdong Province 2020B010188001
6 · The paper itself

Abstract

backgroundSteatosis and inflammation are the hallmarks of nonalcoholic steatohepatitis (NASH). Rotundic acid (RA) is among the key triterpenes of Ilicis Rotundae Cortex and has exhibited multipronged effects in terms of lowering the lipid content and alleviating inflammation. The study objective is to systematically evaluate the potential mechanisms through which RA affects the development and progression of NASH.

methodsTranscriptomic and proteomic analyses of primary hepatocytes isolated from the control, high-fat diet-induced NASH, and RA treatment groups were performed through Gene Ontology analysis and pathway enrichment. Hub genes were identified through network analysis. Integrative analysis revealed key RA-regulated pathways, which were verified by gene and protein expression studies and cell assays.

resultsHub genes were identified and enriched in the Toll-like receptor 4 (TLR4)/activator protein-1 (AP1) signaling pathway and glycolysis pathway. RA reversed glycolysis and attenuated the TLR4/AP1 pathway, thereby reducing lipid accumulation and inflammation. Additionally, lactate release in L-02 cells increased with NaAsO

conclusionsRA is effective in lowering the lipid content and reducing inflammation in mice with NASH by ameliorating glycolysis and TLR4/AP1 pathways, which contributes to the existing knowledge and potentially sheds light on the development of therapeutic interventions for patients with NASH.

Indexed as

Non-alcoholic Fatty Liver DiseaseTriterpenesAnimalsHumansInflammationLipidsLiverMiceMice, Inbred C57BLProteomicsSignal TransductionToll-Like Receptor 4Transcription Factor AP-1Lipidsrotundic acidTLR4 protein, humanToll-Like Receptor 4Transcription Factor AP-1TriterpenesGlycolysisNASHProteomicsRATLR4/AP1Transcriptomics

Identifiers

PMID38049817
PMCPMC10694891
OpenAlexW4389311811

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.