Evidence map›Paper›PMID 38049453›Full record

ArticleScientific reports2023

Metagenomic sequencing detects human respiratory and enteric viruses in air samples collected from congregate settings.

Nicholas R Minor, Mitchell D Ramuta, Miranda R Stauss, Olivia E Harwood, Savannah F Brakefield, Alexandra Alberts, William C Vuyk, Max J Bobholz, Jenna R Rosinski, Sydney Wolf and 6 more

Erratum issuedOpen access · goldAbstract read
In one paragraph

Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
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  5. Review
  6. Article
  7. Article
  8. Article
  9. Shotgun metagenomics on indoor air for surveillance of respiratory, enteric, and skin viruses in a Belgian daycare setting, January to December 2022.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2025
    Article
  10. Observational
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 3 institutions in 1 country.

Nicholas R Minor *Wisconsin National Primate Research Center, Madison, WI, USA.
Mitchell D Ramuta *Department of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Miranda R StaussWisconsin National Primate Research Center, Madison, WI, USA.
Olivia E HarwoodDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Savannah F BrakefieldDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Alexandra AlbertsDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
William C VuykDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Max J BobholzDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Jenna R RosinskiDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Sydney WolfDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Madelyn LundDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Madison MussaDepartment of Pathology and Laboratory Medicine, University of Wisconsin-Madison, 555 Science Drive, Madison, WI, 53711, USA.
Lucas J BeversdorfCity of Milwaukee Health Department Laboratory, Milwaukee, WI, USA.
Matthew T AliotaDepartment of Veterinary and Biomedical Sciences, University of Minnesota, Twin Cities, Minneapolis, MN, USA.
Shelby L O'ConnorWisconsin National Primate Research Center, Madison, WI, USA.
David H O'ConnorWisconsin National Primate Research Center, Madison, WI, USA. dhoconno@wisc.edu.
University of Wisconsin–Madison · USMilwaukee Health Department · USUniversity of Minnesota · US

Funding

Microbial Pathogenesis &Host Responses Training ProgramT32AI055397 · NIAID · UNIVERSITY OF WISCONSIN-MADISON · PI KLEIN, BRUCE STEVEN · 2003 to 2024
$5.5M
NIAID NIH HHS T32 AI055397NIH HHS AAL4371
6 · The paper itself

Abstract

Innovative methods for evaluating virus risk and spread, independent of test-seeking behavior, are needed to improve routine public health surveillance, outbreak response, and pandemic preparedness. Throughout the COVID-19 pandemic, environmental surveillance strategies, including wastewater andair sampling, have been used alongside widespread individual-based SARS-CoV-2 testing programs to provide population-level data. These environmental surveillance strategies have predominantly relied on pathogen-specific detection methods to monitor viruses through space and time. However, this provides a limited picture of the virome present in an environmental sample, leaving us blind to most circulating viruses. In this study, we explore whether pathogen-agnostic deep sequencing can expand the utility of air sampling to detect many human viruses. We show that sequence-independent single-primer amplification sequencing of nucleic acids from air samples can detect common and unexpected human respiratory and enteric viruses, including influenza virus type A and C, respiratory syncytial virus, human coronaviruses, rhinovirus, SARS-CoV-2, rotavirus, mamastrovirus, and astrovirus.

Indexed as

COVID-19EnterovirusEnterovirus InfectionsInfluenza A virusInfluenza, HumanRNA VirusesCOVID-19 TestingHumansPandemicsSARS-CoV-2

Identifiers

PMID38049453
PMCPMC10696062
OpenAlexW4389298363

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.