Evidence map›Paper›PMID 38045421›Full record

ArticlebioRxiv : the preprint server for biology2023

Versatile toolkit for highly-efficient and scarless overexpression of circular RNAs.

Brett W Stringer, Marta Gabryelska, Shashikanth Marri, Letitia Clark, He Lin, Laura Gantley, Ryan Liu, Jeremy E Wilusz, Vanessa M Conn, Simon J Conn

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Brett W StringerCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.ORCID 0000-0003-4552-9736
Marta GabryelskaCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.ORCID 0000-0002-4433-282X
Shashikanth MarriCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.ORCID 0000-0002-0347-8670
Letitia ClarkCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.
He LinCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.
Laura GantleyCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.
Ryan LiuCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.
Jeremy E WiluszVerna and Marrs McLean Department of Biochemistry and Molecular Pharmacology, Therapeutic Innovation Center, Baylor College of Medicine, Houston, TX, 77030, USA.ORCID 0000-0001-6929-8755
Vanessa M ConnCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.
Simon J ConnCollege of Medicine and Public Health, Flinders University, Bedford Park, South Australia, 5032, Australia.ORCID 0000-0002-1376-4515
Flinders University · AUAustralian Centre for Plant Functional Genomics · AUBaylor College of Medicine · US

Funding

Regulatory roles for the Integrator complex and circular RNAsR35GM119735 · NIGMS · UNIVERSITY OF PENNSYLVANIA · PI WILUSZ, JEREMY E · 2016 to 2025
$4.1M
NIGMS NIH HHS R35 GM119735
6 · The paper itself

Abstract

Circular RNAs (circRNAs) are a class of single-stranded, covalently closed RNA that contain a unique back-splice junction (bsj) sequence created by the ligation of their 5' and 3' ends via spliceosome-catalyzed back-splicing. A key step in illuminating the cellular roles of specific circRNAs is via increasing their expression. This is frequently done by transfecting cells with plasmid DNA containing cloned exons from which the circRNA is transcribed, flanked by sequences that promote back-splicing. We observed that commonly used plasmids lead to the production of circRNAs with molecular scars at the circRNA bsj. Stepwise redesign of the cloning vector corrected this problem, ensuring

Identifiers

PMID38045421
PMCPMC10690289
OpenAlexW4388893449

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.