Evidence map›Paper›PMID 38045402›Full record

ArticlebioRxiv : the preprint server for biology2023

Repeat mediated excision of gene drive elements for restoring wild-type populations.

Pratima R Chennuri, Josef Zapletal, Raquel D Monfardini, Martial Loth Ndeffo-Mbah, Zach N Adelman, Kevin M Myles

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Pratima R ChennuriDepartment of Entomology and AgriLife Research, Texas A&M University, College Station, TX 77843, USA.
Josef ZapletalDepartment of Industrial and Systems Engineering, Texas A&M University, College Station, TX 77843, USA.
Raquel D MonfardiniDepartment of Entomology and AgriLife Research, Texas A&M University, College Station, TX 77843, USA.
Martial Loth Ndeffo-MbahDepartment of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USA.
Zach N AdelmanDepartment of Entomology and AgriLife Research, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0001-5901-7171
Kevin M MylesDepartment of Entomology and AgriLife Research, Texas A&M University, College Station, TX 77843, USA.ORCID 0000-0002-9820-2502
Texas A&M University System · USTexas A&M University · US

Funding

Self-Eliminating Strategy To Control Gene DriveR01AI148787 · NIAID · TEXAS A&M AGRILIFE RESEARCH · PI Zach N. Adelman, KEVIN M MYLES · 2020 to 2026
$4.4M
Hierarchical antiviral immunity in disease vector mosquitoesR01AI119081 · NIAID · VIRGINIA POLYTECHNIC INST AND ST UNIV · PI MYLES, KEVIN M · 2015 to 2019
$1.9M
NIAID NIH HHS R01 AI119081NIAID NIH HHS R01 AI148787
6 · The paper itself

Abstract

We demonstrate here that single strand annealing (SSA) repair can be co-opted for the precise autocatalytic excision of a drive element. Although SSA is not the predominant form of DNA repair in eukaryotic organisms, we increased the likelihood of its use by engineering direct repeats at sites flanking the drive allele, and then introducing a double-strand DNA break (DSB) at a second endonuclease target site encoded within the drive allele. We have termed this technology Repeat Mediated Excision of a Drive Element (ReMEDE). Incorporation of ReMEDE into the previously described mutagenic chain reaction (MCR) gene drive, targeting the

Indexed as

autonomousCas9CRISPRDirect RepeatsDNA Repairdouble-strand breakDrosophilaGene drivehomingHomologous RecombinationHRSingle Strand AnnealingSSA

Identifiers

PMID38045402
PMCPMC10690251
OpenAlexW4389055796

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.