Evidence map›Paper›PMID 38041745›Full record

ArticleFolia microbiologica2024

A recombinase polymerase amplification-SYBR Green I assay for the rapid and visual detection of Brucella.

Jiang Chang, Nan Wang, Jun-Peng Zhan, Shi-Jun Zhang, De-Ying Zou, Feng Li, Ying Zhang, Yan-Song Li, Pan Hu, Shi-Ying Lu and 2 more

Abstract read
PubMed Publisher
In one paragraph

Article in Folia microbiologica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Jiang ChangState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Nan WangJilin Academy of Animal Husbandry and Veterinary Medicine, Changchun, China.
Jun-Peng ZhanState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Shi-Jun ZhangState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
De-Ying ZouState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Feng LiState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Ying ZhangState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Yan-Song LiState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Pan HuState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Shi-Ying LuState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Zeng-Shan LiuState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China.
Hong-Lin RenState Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, Institute of Zoonosis, College of Veterinary Medicine, College of Animal Sciences, Jilin University, Changchun, China. renhl@jlu.edu.cn.ORCID http://orcid.org/0000-0002-3364-8335
Jilin University · CNBinzhou Medical University · CNDalian University of Foreign Languages · CN

Funding

Jilin Scientific and Technological Development Program 20200402059NC
6 · The paper itself

Abstract

Brucellosis is a zoonosis caused by Brucella, which poses a great threat to human health and animal husbandry. Pathogen surveillance is an important measure to prevent brucellosis, but the traditional method is time-consuming and not suitable for field applications. In this study, a recombinase polymerase amplification-SYBR Green I (RPAS) assay was developed for the rapid and visualized detection of Brucella in the field by targeting BCSP31 gene, a conserved marker. The method was highly specific without any cross-reactivity with other common bacteria and its detection limit was 2.14 × 10

Indexed as

BrucellaBrucellosisNucleic Acid Amplification TechniquesRecombinasesAnimalsCattleDNA, BacterialHumansLimit of DetectionMilkSensitivity and SpecificityDNA, BacterialRecombinasesBCSP31 geneBrucellaRecombinase polymerase amplificationSYBR Green IVisualization

Identifiers

PMID38041745
OpenAlexW4389274126

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.