ArticleInternational journal of fertility & sterility2023
The Decellularized Calf Testis: Introducing Suitable Scaffolds for Spermatogenesis Studies.
Article in International journal of fertility & sterility, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Decellularization method and tissue region impact the structural properties and composition of porcine vaginal extracellular matrix.Acta biomaterialia · 2026Article
- 3D bioprinting innovations: a new frontier in breast cancer research.Medical oncology (Northwood, London, England) · 2025Review
- Innovation in ovary decellularization methods: Chemical and herbal detergents.Regenerative therapy · 2025Article
- Understanding the role of electrostatic force, van der Waals force, and osmotic pressure in retinal function and barrier integrity.International journal of retina and vitreous · 2025Review
- Cryopreservation and culture strategies for testicular tissue and cells in small and large animals.Frontiers in veterinary science · 2025Review
- Engineering a 3D platform for testis bioengineering: generation and proteomic profiling of decellularized fish testicular scaffolds.Frontiers in bioengineering and biotechnology · 2025Article
- Testicular extracellular matrix/gelatin-based scaffold using gas foaming to support spermatogonial stem cells.Iranian journal of basic medical sciences · 2025Article
- Review
- Decellularized kidney capsule as a three-dimensional scaffold for tissue regeneration.Cell and tissue banking · 2024Article
- Generation of bovine decellularized testicular bio-scaffolds as a 3D platform for testis bioengineering.Frontiers in bioengineering and biotechnology · 2024Article
- Nerve regeneration using decellularized tissues: challenges and opportunities.Frontiers in neuroscience · 2023Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMen's infertility and lack of production of healthy and active sperm are concerns of recent years in most countries. Studies on the preparation of extracellular matrix (ECM) from decellularization of testis tissue and spermatogenesis could provide proper results to solve some of the men's infertility problems. This study aims to decellularize calf testis by different methods to reach a suitable scaffold and introduce it in spermatogenesis studies. MATERIALS AND
methodsIn this experimental study, calf testis were decellularized by a freeze-de freeze, 1% sodium deoxycholate (SD), 0.1% sodium dodecyl sulfate (SDS), 0.1% SDS-vacuum, 1% SDS, 1% SDS-vacuum, and Triton- X100 methods. The content of DNA, collagen, and glycosaminoglycan (GAG) was analyzed using the kit and staining with Hematoxylin-Eosin, Masson's trichrome, Alcian blue, and Orcein methods. The morphology of the scaffolds was analyzed with a scanning electron microscope (SEM).
resultsMethods of 1% SDS, 1% SDS-vacuum, and 1% SD completely removed the cells. The preservation of collagen and GAG was confirmed using the staining kit and methods. The use of a vacuum showed greater porosity in the SEM images. Toxicity and hemolysis were not observed in the scaffolds.
conclusionTestis decellularization with 1% SDS and 1% SD, in addition to cell removal, could maintain the ECM structure to a large extent without having cytotoxic and hemolysis effects.
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