ArticleBMC plant biology2023
Functional and kinetics of two efficient phenylalanine ammonia lyase from Pyrus bretschneideri.
Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 20 citations in OpenAlex.
- Genome-wide analysis and expression profiling of the phenylalanine ammonia-lyase gene family in Chrysanthemum morifolium.BMC plant biology · 2026Article
- Recent Advances in Physiological and Biochemical Responses of Grapevines to Downy Mildew Infection.Plants (Basel, Switzerland) · 2026Review
- Multi-omics analysis reveals flavor biosynthesis in Camellia seed oil: phenylpropanoid pathway activation and antioxidant modulation of lipoxygenase (LOX).Food chemistry: X · 2026Article
- Role of Peat Moss (Sphagnum) in Reduction of Environmental Toxicity in Correlation with Various Microbes.Applied biochemistry and biotechnology · 2025Review
- Optimizing sweet fennel growth and quality: the impact of cobalt supplement on vegetative growth, yield, and chemical composition.BMC plant biology · 2025Article
- Article
- Gap-free genome assemblies of two Pyrus bretschneideri cultivars and GWAS analyses identify a CCCH zinc finger protein as a key regulator of stone cell formation in pear fruit.Plant communications · 2025Article
- Biochemical evaluation of molecular parts for flavonoid production using plant synthetic biology.Frontiers in plant science · 2025Review
- Analysis of maize PAL pan gene family and expression pattern under lepidopteran insect stress.Frontiers in plant science · 2025Article
- Transcriptome Analysis Reveals Key Pathways and Genes Involved in Lodging Resistance of Upland Cotton.Plants (Basel, Switzerland) · 2024Article
- Deciphering High-Temperature-Induced Lignin Biosynthesis in Wheat through Comprehensive Transcriptome Analysis.Plants (Basel, Switzerland) · 2024Article
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
backgroundThe enzyme phenylalanine ammonia lyase (PAL) controls the transition from primary to secondary metabolism by converting L-phenylalanine (L-Phe) to cinnamic acid. However, the function of PAL in pear plants (Pyrus bretschneideri) has not yet been fully elucidated.
resultsWe identified three PAL genes (PbPAL1, PbPAL2 and PbPAL3) from the pear genome by exploring pear genome databases. The evolutionary tree revealed that three PbPALs were classified into one group. We expressed PbPAL1 and PbPAL2 recombinant proteins, and the purified PbPAL1 and PbPAL2 proteins showed strict substrate specificity for L-Phe, no activity toward L-Tyr in vitro, and modest changes in kinetics and enzyme characteristics. Furthermore, overexpression of PbAL1 and PbPAL1-RNAi, respectively, and resulted in significant changes in stone cell and lignin contents in pear fruits. The results of yeast one-hybrid (Y1H) assays that PbWLIM1 could bind to the conserved PAL box in the PbPAL promoter and regulate the transcription level of PbPAL2.
conclusionsOur findings not only showed PbPAL's potential role in lignin biosynthesis but also laid the foundation for future studies on the regulation of lignin synthesis and stone cell development in pear fruit utilizing molecular biology approaches.
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