Evidence map›Paper›PMID 38040876›Full record

ArticleChinese journal of integrative medicine2024

Artemisia vulgaris Induces Tumor-Selective Ferroptosis and Necroptosis via Lysosomal Ca

Lucas Dos Santos Zamarioli, Michele Rosana Maia Santos, Adolfo Garcia Erustes, Vanessa Marques Meccatti, Thaís Cristine Pereira, Soraya S Smaili, Maria Cristina Marcucci, Carlos Rocha Oliveira, Gustavo J S Pereira, Claudia Bincoletto

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In one paragraph

Article in Chinese journal of integrative medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Role of Kinin BCellular and molecular neurobiology · 2026
    Article
  2. [Research progress of iron metabolism and ferroptosis in myeloid neoplasms].Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2024
    Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Lucas Dos Santos ZamarioliDepartment of Pharmacology, Paulista School of Medicine, Federal University of São Paulo, São Paulo, SP, 04044-020, Brazil.
Michele Rosana Maia SantosDepartment of Pharmacology, Paulista School of Medicine, Federal University of São Paulo, São Paulo, SP, 04044-020, Brazil.
Adolfo Garcia ErustesDepartment of Pharmacology, Paulista School of Medicine, Federal University of São Paulo, São Paulo, SP, 04044-020, Brazil.
Vanessa Marques MeccattiDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (ICT-UNESP), São Paulo, SP, 12231-280, Brazil.
Thaís Cristine PereiraDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (ICT-UNESP), São Paulo, SP, 12231-280, Brazil.
Soraya S SmailiDepartment of Pharmacology, Paulista School of Medicine, Federal University of São Paulo, São Paulo, SP, 04044-020, Brazil.
Maria Cristina MarcucciDepartment of Biosciences and Oral Diagnosis, Institute of Science and Technology, São Paulo State University (ICT-UNESP), São Paulo, SP, 12231-280, Brazil.
Carlos Rocha OliveiraResearch Group on Phytocomplexes and Cell Signaling, School of Health Sciences, Anhembi Morumbi University, São Paulo, SP, 03164-000, Brazil.
Gustavo J S PereiraDepartment of Pharmacology, Paulista School of Medicine, Federal University of São Paulo, São Paulo, SP, 04044-020, Brazil.
Claudia BincolettoDepartment of Pharmacology, Paulista School of Medicine, Federal University of São Paulo, São Paulo, SP, 04044-020, Brazil. claudia.bincoletto@unifesp.br.
Universidade Federal de São Paulo · BRUniversidade Estadual Paulista (Unesp) · BRAnhembi Morumbi University · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo evaluate the chemical composition and effects of Artemisia vulgaris (AV) hydroalcoholic extract (HEAV) on breast cancer cells (MCF-7 and SKBR-3), chronic myeloid leukemia (K562) and NIH/3T3 fibroblasts.

methodsPhytochemical analysis of HEAV was done by high-performance liquid chromatography-mass (HPLC) spectrometry. Viability and cell death studies were performed using trypan blue and Annexin/FITC-7AAD, respectively. Ferrostatin-1 (Fer-1) and necrostatin-1 (Nec-1) were used to assess the mode of HEAV-induced cell death and acetoxymethylester (BAPTA-AM) was used to verify the involvement of cytosolic calcium in this event. Cytosolic calcium measurements were made using Fura-2-AM.

resultsHEAV decreased the viability of MCF-7, SKBR-3 and K562 cells (P<0.05). The viability of HEAV-treated K562 cells was reduced compared to HEAV-exposed fibroblasts (P<0.05). Treatment of K562 cells with HEAV induced cell death primarily by late apoptosis and necrosis in assays using annexin V-FITC/7-AAD (P<0.05). The use of Nec-1 and Fer-1 increased the viability of K562 cells treated with HEAV relative to cells exposed to HEAV alone (P<0.01). HEAV-induced Ca

conclusionsHEAV is cytotoxic and activates several modalities of cell death, which are partially dependent on lysosomal release of Ca

Indexed as

ArtemisiaCalcium SignalingCell SurvivalFerroptosisLysosomesNecroptosisPlant ExtractsAnimalsCalciumCell Line, TumorHumansK562 CellsMiceNIH 3T3 CellsCalciumPlant ExtractsArtemisia vulgarisCa2+ signalingferroptosislate apoptosisnecroptosis

Identifiers

PMID38040876
OpenAlexW4389257561

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.