Evidence map›Paper›PMID 38038849›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Motility Assay to Probe the Calcium Sensitivity of Myosin and Regulated Thin Filaments.

Chao Liu, Kathleen M Ruppel, James A Spudich

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
4.1field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Chao LiuDepartment of Biochemistry, Beckman Center B405, Stanford University School of Medicine, Stanford, CA, USA.
Kathleen M RuppelDepartment of Biochemistry, Beckman Center B405, Stanford University School of Medicine, Stanford, CA, USA. kruppel@stanford.edu.
James A SpudichDepartment of Biochemistry, Beckman Center B405, Stanford University School of Medicine, Stanford, CA, USA. jspudich@stanford.edu.
Stanford University · USLawrence Livermore National Laboratory · US

Funding

MYOSIN MOVEMENT IN VITRO: MOLECULAR CHARACTERIZATIONR01GM033289 · NIGMS · STANFORD UNIVERSITY · PI SPUDICH, JAMES · 1985 to 2024
$13.6M
From proteins to cells to tissues: A multi-scale assessment of biomechanical regulation by the myosin molecular motorRM1GM131981 · NIGMS · STANFORD UNIVERSITY · PI BERNSTEIN, DANIEL, MACK, DAVID LEE · 2019 to 2023
$10.3M
Molecular Characterization of Cardiomyopathy Mutations in Human Cardiac MyosinR01HL117138 · NHLBI · UNIVERSITY OF COLORADO · PI LEINWAND, LESLIE ANNE, PERKINS, THOMAS TUPPER · 2013 to 2021
$4.2M
Shared TIRF and Patterned Illumination Fluorescence MicroscopeS10RR026775 · NCRR · STANFORD UNIVERSITY · PI STRAIGHT, AARON F · 2010 to 2010
$465k
NCRR NIH HHS S10 RR026775NHLBI NIH HHS R01 HL117138NIGMS NIH HHS R01 GM033289NIGMS NIH HHS RM1 GM131981
6 · The paper itself

Abstract

Calcium-dependent activation of the thin filament mediated by the troponin-tropomyosin complex is key in the regulation of actin-myosin based muscle contraction. Perturbations to this system, either physiological (e.g., phosphorylation of myosin light chains) or pathological (e.g., mutations that cause familial cardiomyopathies), can alter calcium sensitivity and thus have important implications in human health and disease. The in vitro motility assay provides a quantitative and precise method to study the calcium sensitivity of the reconstituted myosin-thin filament motile system. Here we present a simple and robust protocol to perform calcium-dependent motility of β-cardiac myosin and regulated thin filaments. The experiment is done on a multichannel microfluidic slide requiring minimal amounts of proteins. A complete velocity vs. calcium concentration curve is produced from one experiment in under 1 h.

Indexed as

CalciumMyosinsActin CytoskeletonActinsHumansMuscle ContractionTropomyosinActinsCalciumMyosinsTropomyosinCalcium regulationFilament trackingFluorescence microscopyIn vitro motilityMicrofluidicsMyosinRegulated thin filamentTemperature dependence

Identifiers

PMID38038849
PMCPMC10773985
OpenAlexW4389226816

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.