ArticleMethods in molecular biology (Clifton, N.J.)2024
Quantification of Fentanyl and Norfentanyl in Whole Blood Using Liquid Chromatography-Tandem Mass Spectrometry.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed, 3 citations in OpenAlex.
- Interpol review of forensic drug chemistry, 2022-2025.Forensic science international. Synergy · 2026Review
- Exposures to synthetic cathinones, fentanyl, and xylazine among nightclub attendees in New York City, 2024.Drug and alcohol dependence · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fentanyl is a synthetic opioid used in pain management with a potency 50-100 times that of morphine. Due to fentanyl's high potency, very low dosages are needed to elicit the desired response. Fentanyl is gaining popularity as a drug of abuse. Overdose of fentanyl causes respiratory depression that can lead to death. Fentanyl undergoes N-dealkylation in the liver to its inactive metabolite norfentanyl. Quantitation of fentanyl and its metabolite norfentanyl in whole blood can be performed using liquid chromatography-mass spectrometry. In this method, whole blood samples are spiked with deuterated internal standards for fentanyl and norfentanyl. The samples are alkalized with potassium hydroxide and the drugs are extracted with an organic solvent. Extracts are dried and reconstituted, then injected on LC-MS/MS. They are quantitated using positive ion multiple reaction monitoring (MRM) mode.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.