Evidence map›Paper›PMID 38036045›Full record

ArticleCanadian journal of ophthalmology. Journal canadien d'ophtalmologie2025

Multicentre analysis of nucleic acid quantification using aqueous humour liquid biopsy in uveal melanoma: implications for clinical testing.

Sarah B Pike, Mark W Reid, Chen-Ching Peng, Christina Chang, Benjamin Y Xu, Dan S Gombos, Sapna Patel, Liya Xu, Jesse L Berry

Open access · greenAbstract readMulticenter Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Canadian journal of ophthalmology. Journal canadien d'ophtalmologie, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Article
  3. Role of miRNA in adult ocular tumorigenesis.Frontiers in molecular biosciences · 2025
    Review
  4. Review
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Sarah B PikeUSC Roski Eye Institute, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA; Vision Center, Children's Hospital Los Angeles, Los Angeles, CA.
Mark W ReidVision Center, Children's Hospital Los Angeles, Los Angeles, CA; Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA.
Chen-Ching PengUSC Roski Eye Institute, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA; Vision Center, Children's Hospital Los Angeles, Los Angeles, CA; Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA.
Christina ChangUSC Roski Eye Institute, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA.
Benjamin Y XuUSC Roski Eye Institute, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA.
Dan S GombosSection of Ophthalmology, Department of Head and Neck Surgery, Division of Surgery, University of Texas MD Anderson Cancer Center, Houston, TX.
Sapna PatelDepartment of Melanoma Medical Oncology, University of Texas MD Anderson Cancer Center, Houston, TX.
Liya XuUSC Roski Eye Institute, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA; Vision Center, Children's Hospital Los Angeles, Los Angeles, CA; Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA.
Jesse L BerryUSC Roski Eye Institute, Keck School of Medicine, University of Southern California (USC), Los Angeles, CA; Vision Center, Children's Hospital Los Angeles, Los Angeles, CA; Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA; Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California, Los Angeles, CA. Electronic address: Jesse.Berry@med.usc.edu.
Children's Hospital of Los Angeles · USSouthern California Eye Institute · USThe University of Texas MD Anderson Cancer Center · US

Funding

Development of a surrogate liquid biopsy from the aqueous humor in retinoblastoma eyes.K08CA232344 · NCI · CHILDREN'S HOSPITAL OF LOS ANGELES · PI BERRY, JESSE L · 2018 to 2022
$1.1M
NCI NIH HHS K08 CA232344NCI NIH HHS R01CA282759)
6 · The paper itself

Abstract

objectiveUveal melanoma (UM) tumour biopsy is limited by size and intratumour heterogeneity. We explored the potential of aqueous humour (AH) liquid biopsy for UM by quantifying analytes in samples collected at diagnosis and after brachytherapy to look for clinical correlations with tumour features.

designCase-series study.

participantsSixty-six UM patients and 16 control subjects from a tertiary care hospital.

methodsThe study included 119 UM AH samples and 16 control samples analyzed for unprocessed analytes (i.e., dsDNA, miRNA, and protein) using Qubit fluorescence assays.

resultsAnalytes were widely quantifiable among available UM AH samples (dsDNA: 94.1%; miRNA: 88.0%; protein: 95.2%) at significantly higher concentrations than among control samples (dsDNA, p = 0.008; miRNA, p < 0.0001; protein, p = 0.007). In samples taken at diagnosis, concentrations were higher at more advanced American Joint Cancer Commission stages; when comparing most advanced stage III with least advanced stage I, median dsDNA was 4 times greater (p < 0.0001), miRNA was 2 times greater (p = 0.001), and protein was 3 times greater (p < 0.0001). Analytes were quantifiable in >70% of diagnostic samples from eyes with tumours <2 mm tall. Height had a positive association with diagnostic analyte concentrations (dsDNA: R = 0.43, p = 0.0007; miRNA: R = 0.35, p = 0.01; protein: R = 0.39, p = 0.005). Samples taken after brachytherapy showed significantly higher concentrations than diagnostic samples (p < 0.01 for all).

conclusionsUM AH is a rich repository of analytes. Samples from eyes with more advanced stage and larger tumours had higher concentrations, though analytes also were quantifiable in eyes with smaller, less advanced tumours. Future analysis of AH analytes may be informative in the pursuit of personalized UM treatments.

Indexed as

Aqueous HumorBiomarkers, TumorDNA, NeoplasmMelanomaUveal NeoplasmsAdultAgedAged, 80 and overBrachytherapyFemaleHumansLiquid BiopsyMaleMiddle AgedNeoplasm StagingUveal MelanomaBiomarkers, TumorDNA, Neoplasm

Identifiers

PMID38036045
PMCPMC11128479
OpenAlexW4389042641

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.