ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2024
Construction of Host Plant Insect-Resistance Mutant Library by High-Throughput CRISPR/Cas9 System and Identification of A Broad-Spectrum Insect Resistance Gene.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 40 citations in OpenAlex.
- Novel effector HYPB1 of cotton bollworm (Helicoverpa armigera) inhibits biosynthesis of plant secondary metabolites and promotes feeding by targeting cotton dirigent protein GhDIR15.Journal of integrative plant biology · 2026Article
- A small-scale CRISPR mutant library in rapeseed of commercial cultivar Zhongshuang 11.Horticulture research · 2026Article
- Three Decades of China's Bt Cotton: Achievements and Insights.Plant biotechnology journal · 2026Review
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Accelerating Resistance Breeding: Emerging Methods to Identify and Validate Plant Immunity Genes.Plants (Basel, Switzerland) · 2026Review
- Article
- Breeding jassid-resistant okra (Frontiers in plant science · 2026Review
- Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution.Journal of advanced research · 2026Article
- Genotyping by sequencing reveals a chromosome A04 QTL governing whitefly resistance in upland cotton.Scientific reports · 2025Article
- The Mitochondrial Protein RESISTANCE to APHIDS 9 Interacts with S40 to Resist Aphid Infestation by Modulating Reactive Oxygen Species Homeostasis in Maize (Zea mays).Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A CRISPR-Cas9 library to target putative redundant gene sets facilitates their functional exploration in grain development in rice.BMC plant biology · 2025Article
- Strategies for plant-virus disease management from gene editing to nanotechnology.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Review
- Deciphering the dynamic expression network of fiber elongation and the functional role of the GhTUB5 gene for fiber length in cotton based on an introgression population of upland cotton.Journal of advanced research · 2025Article
- Large-Scale Rice Mutant Establishment and High-Throughput Mutant Manipulation Help Advance Rice Functional Genomics.Plants (Basel, Switzerland) · 2025Review
- Construction of a Cas9-targeted mutagenesis mini-library in the upland cotton genetic standard line (TM-1).Plant cell reports · 2025Article
- CRISPR-Cas9-mediated construction of a cotton CDPK mutant library for identification of insect-resistance genes.Plant communications · 2024Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Advances in Cotton Genomics, Genetics and Breeding.Plants (Basel, Switzerland) · 2024Article
- Precise fine-turning of GhTFL1 by base editing tools defines ideal cotton plant architecture.Genome biology · 2024Article
- Advances in genome sequencing and artificially induced mutation provides new avenues for cotton breeding.Frontiers in plant science · 2024Review
Corrections and comments
- Erratum issued
Authors and funding
29 authors at 6 institutions in 3 countries.
Funding
Abstract
Insects pose significant challenges in cotton-producing regions. Here, they describe a high-throughput CRISPR/Cas9-mediated large-scale mutagenesis library targeting endogenous insect-resistance-related genes in cotton. This library targeted 502 previously identified genes using 968 sgRNAs, generated ≈2000 T0 plants and achieved 97.29% genome editing with efficient heredity, reaching upto 84.78%. Several potential resistance-related mutants (10% of 200 lines) their identified that may contribute to cotton-insect molecular interaction. Among these, they selected 139 and 144 lines showing decreased resistance to pest infestation and targeting major latex-like protein 423 (GhMLP423) for in-depth study. Overexpression of GhMLP423 enhanced insect resistance by activating the plant systemic acquired resistance (SAR) of salicylic acid (SA) and pathogenesis-related (PR) genes. This activation is induced by an elevation of cytosolic calcium [Ca
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.