Evidence map›Paper›PMID 38027705›Full record

ArticleHeliyon2023

Proof of concept: Pull down assay using bovine serum albumin and an immunomodulator small molecule.

Yasser Tabana, Chih-Hsuan Lin, Dinesh Babu, Ramanaguru Siva-Piragasam, Ashley A Ponich, Tae Chul Moon, Arno G Siraki, Shokrollah Elahi, Richard Fahlman, Frederick G West and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 2 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Yasser TabanaFaculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2H1, Canada.
Chih-Hsuan LinDepartment of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
Dinesh BabuLi Ka Shing Applied Virology Institute, University of Alberta, Edmonton, AB, T6G 2E1, Canada.
Ramanaguru Siva-PiragasamDepartment of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada.
Ashley A PonichDepartment of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
Tae Chul MoonFaculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2H1, Canada.
Arno G SirakiFaculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2H1, Canada.
Shokrollah ElahiSchool of Dentistry, University of Alberta, Edmonton, AB, T6G 1C9, Canada.
Richard FahlmanDepartment of Biochemistry, University of Alberta, Edmonton, AB, T6G 2H7, Canada.
Frederick G WestDepartment of Chemistry, University of Alberta, Edmonton, AB, T6G 2G2, Canada.
Khaled BarakatFaculty of Pharmacy and Pharmaceutical Sciences, University of Alberta, Edmonton, AB, T6G 2H1, Canada.
University of Alberta · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the past decade, there has been increasing interest in use of small molecules for immunomodulation. The affinity-based pull-down purification is an essential tool for target identification of small molecules and drug discovery. This study presents our recent efforts to investigate the cellular target(s) of Compound A, a small molecule with demonstrated immunomodulatory properties in human peripheral blood mononuclear cells (PBMCs). While we have previously observed the immunomodulatory activity of Compound A in PBMCs, the specific molecular targets underlying its effects remains elusive. To address this challenge, we synthesized a trifluoromethyl phenyl diazirine (TPD)-bearing trifunctional Probe

Identifiers

PMID38027705
PMCPMC10651465
OpenAlexW4388009503

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.