Evidence map›Paper›PMID 38027571›Full record

ArticleHeliyon2023

Evaluation of next generation sequencing approaches for SARS-CoV-2.

Valentina Curini, Massimo Ancora, Lucija Jurisic, Valeria Di Lollo, Barbara Secondini, Luana Fiorella Mincarelli, Marialuigia Caporale, Ilaria Puglia, Luigina Di Gialleonardo, Iolanda Mangone and 5 more

Abstract read
In one paragraph

Article in Heliyon, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Microorganisms · 2025
    Article
  3. Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Valentina CuriniIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Massimo AncoraIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Lucija JurisicIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Valeria Di LolloIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Barbara SecondiniIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Luana Fiorella MincarelliIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Marialuigia CaporaleIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Ilaria PugliaIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Luigina Di GialleonardoIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Iolanda MangoneIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Marco Di DomenicoIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Adriano Di PasqualeIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Alessio LorussoIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Maurilia MarcacciIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.
Cesare CammàIstituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise, Teramo, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Within public health control strategies for SARS-CoV-2, whole genome sequencing (WGS) is essential for tracking viral spread and monitoring the emergence of variants which may impair the effectiveness of vaccines, diagnostic methods, and therapeutics. In this manuscript different strategies for SARS-CoV-2 WGS including metagenomic shotgun (SG), library enrichment by myBaits® Expert Virus-SARS-CoV-2 (Arbor Biosciences), nCoV-2019 sequencing protocol, ampliseq approach by Swift Amplicon® SARS-CoV-2 Panel kit (Swift Biosciences), and Illumina COVIDSeq Test (Illumina Inc.), were evaluated in order to identify the best approach in terms of results, labour, and costs. The analysis revealed that Illumina COVIDSeq Test (Illumina Inc.) is the best choice for a cost-effective, time-consuming production of consensus sequences.

Indexed as

ComparisonCostLabor timeMethodsSARS-CoV-2Whole genome sequencing

Identifiers

PMID38027571
PMCPMC10643093

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.