ArticleACS omega2023
Hairpin/DNA Ring Ternary Complex Initiated Rolling Circle Amplification for an Elevated Accuracy and Its Application in Analyzing Let-7a.
Article in ACS omega, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Split G‑Quadruplex-Programmed Fluorescent Platform for Low Background Signal and Sensitive Nonsmall Cell Lung Cancer-Related MicroRNA Analysis.ACS omega · 2026Article
- Garland Rolling Circle Amplification Mediated Self-Priming Extension Strategy for Sensitive and Label-FreeJournal of microbiology and biotechnology · 2026Article
- CRISPR/Cas12a-RCA enables ultrasensitive detection of circulating free DNA for noninvasive diagnosis of echinococcosis.PLoS neglected tropical diseases · 2026Article
- Programmable DNA aptamer logic gates: from structural design to integrated systems for intelligent nanoscale biosensors.Analytical and bioanalytical chemistry · 2025Review
- Single-Step Detection of microRNA via the Programmable DNAzyme Probe-Mediated Multiple Signal Recycling.ACS omega · 2025Article
- Exonuclease-III Assisted Signal Cycle Integrating with Self-Priming Mediated Chain Extension for Sensitive and Reliable MicroRNA Detection.ACS omega · 2025Article
- Glucose Oxidase Coupling with Pistol-Like DNAzyme Based Colorimetric Assay for Sensitive Glucose Detection in Tears and Saliva.Applied biochemistry and biotechnology · 2025Article
- Circle Padlock-Mediated Catalytic Hairpin Assembly Cooperating Primer Exchange Reaction for Sensitive and Label-Free MicroRNA Detection.ACS omega · 2024Article
- Handgrip-Ring Structure Sensing Probe Assisted Multiple Signal Amplification Strategy for Sensitive and Label-Free Single-Stranded Nucleic Acid Analysis.Journal of analytical methods in chemistry · 2024Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rolling circle amplification (RCA) is an attractive isothermal nucleic acid amplification approach and has been widely applied in constructing a variety of biosensors. However, the inevitable drawbacks of lacking enough selectivity greatly hindered further applications of RCA-based approaches. Here, we develop a novel RCA-based approach by integrating the specific target recognition capability of the hairpin/DNA ring ternary complex and multiple signal amplification and successfully applied it for let-7a detection. In this method, let-7a specifically unfolds the hairpin probe (Hp probe) in the ternary complex to induce target recycle and RCA- and DNAzyme-based signal generation. Based on this, the established approach exhibits a high selectivity to let-7a, and the response of the approach to one base pair mismatched sequences was 24.9%, indicating a significantly improved specificity. Meanwhile, the limit of detection is as low as 342 aM, which can meet the high requirement for a trace amount of miRNA detection. In all, we believe that the established approach can offer a new avenue for miRNA detection and post-tumor care.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.