ArticleMethods in molecular biology (Clifton, N.J.)2024
Rapid Identification of Peptide-Receptor-Coreceptor Complexes in Protoplasts.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Secreted signaling peptides, also called peptide hormones, play crucial roles in regulating plant growth, development, and immunity. Plant peptide hormones are perceived by plasma membrane-localized receptor-like kinases (RLKs) or receptor-like proteins (RLPs) that harbor specific extracellular domains to bind and recognize the corresponding peptide ligands. Binding of a peptide ligand to its receptor usually induces the hetero-dimerization of the cognate receptor and a coreceptor, followed by the phosphorylation and activation of the receptor complex to transduce downstream signaling. Therefore, matching peptide ligands with their respective receptors/coreceptors is crucial for elucidating peptide hormone signaling pathways. In this chapter, using the RGF7 peptide-RGI4/RGI5 receptor-BAK1 coreceptor complex as an example, we describe a rapid method to identify the peptide ligand-receptor-coreceptor complexes via co-immunoprecipitation assays using recombinant proteins transiently expressed in Arabidopsis protoplasts.
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