ArticleMethods in molecular biology (Clifton, N.J.)2024
Quantitative Measurement of Pattern-Triggered ROS Burst as an Early Immune Response in Tomato.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Subfamily-level comparative transcriptomics of key immune regulators in plants and suspension cells reveals novel rice blast resistance genes.Plant & cell physiology · 2026Article
- Poltergeist-Like 2 (PLL2)-dependent activation of herbivore defence distinguishes systemin from other immune signalling pathways.Nature plants · 2025Article
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Authors and funding
3 authors.
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Abstract
The rapid accumulation of extracellular "reactive oxygen species" (ROS), also known as the "oxidative burst", is an early plant immune response triggered by pathogen-derived microbe-associated molecular patterns and by endogenous plant signaling molecules. The oxidative burst is often used as a readout for the activation of defense signaling. Here, we present a detailed protocol for the continuous measurement of ROS production in leaf discs of tomato plants, using a chemiluminescence-based assay in a microtiter plate format. We also include recommendations for data analysis and for the quantitative assessment of differences in ROS burst dynamics, as caused by different types of elicitors, or in different tomato genotypes.
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