Evidence map›Paper›PMID 38019432›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

A Quick Method to Analyze Peptide-Regulated Anthocyanin Biosynthesis.

Eric Bühler, Andreas Schaller, Nils Stührwohldt

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Eric BühlerDepartment of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim, Stuttgart, Germany.
Andreas SchallerDepartment of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim, Stuttgart, Germany.
Nils StührwohldtDepartment of Plant Physiology and Biochemistry, Institute of Biology, University of Hohenheim, Stuttgart, Germany. nils.stuehrwohldt@uni-hohenheim.de.
University of Hohenheim · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Post-translationally modified peptides are now recognized as important regulators of plant stress responses. We recently identified the sulfated CLE-LIKE6 (CLEL6) peptide as a negative regulator of anthocyanin biosynthesis in dark-grown and in light-stressed Arabidopsis seedlings. The function of CLEL6 depends on proteolytic processing by subtilisin-like serine proteinase SBT6.1, and on tyrosine sulfation by tyrosylprotein sulfotransferase (TPST), and CLEL6 signaling relies on the ROOT MERISTEM GROWTH FACTOR 1 INSENSITIVE (RGI) receptor family. In this chapter, we describe in detail how to quantify peptide-regulated and stress-induced anthocyanin biosynthesis. We include protocols for peptide treatment of Arabidopsis seedlings and growth under different stress conditions, for the extraction and quantification of anthocyanins, and for the expression analysis of anthocyanin biosynthetic genes.

Indexed as

AnthocyaninsArabidopsisPeptide BiosynthesisPeptidesProteolysisSeedlingsAnthocyaninsPeptidesAnthocyaninscDNA synthesisCLEL peptidesGene expressionLight-stressPhotometric analysisqPCRRNA extractionStress response

Identifiers

PMID38019432
OpenAlexW4389146695

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.