Evidence map›Paper›PMID 38019388›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Isolation of Human Villous Cytotrophoblastic Cells from Term Placenta for Transfection.

Caroline Toudic, Benoit Barbeau

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Caroline ToudicDépartement des sciences biologiques, Centre d'excellence en recherche sur les maladies orphelines-Fondation Courtois, Université du Québec à Montréal, Montreal, QC, Canada.
Benoit BarbeauDépartement des sciences biologiques, Centre d'excellence en recherche sur les maladies orphelines-Fondation Courtois, Université du Québec à Montréal, Montreal, QC, Canada. barbeau.benoit@uqam.ca.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Isolation of villous cytotrophoblastic cells (vCTBs) from placental tissue provides the possibility of studying differentiation of these cells as well as their function. We describe herein a protocol, in which term placentas are used to isolate a population of vCTB in high quantities. These cells are subsequently used to transfect siRNA through microporation and thereby to achieve efficient silencing of targeted genes.

Indexed as

PlacentaTrophoblastsCell DifferentiationEpithelial CellsFemaleHumansPregnancyTransfectionPercoll gradientPlacentasiRNATransfectionVillous cytotrophoblastic cells

Identifiers

PMID38019388

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.