Evidence map›Paper›PMID 38019387›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Isolation and Maintenance in Culture of Primary Human Trophoblast from Term Placentae.

Yohanes N S Nursalim, Katie M Groom, Cherie Blenkiron, Lawrence W Chamley

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yohanes N S NursalimDepartment of Obstetrics and Gynaecology, School of Medicine, University of Auckland, Auckland, New Zealand. y.nursalim@auckland.ac.nz.
Katie M GroomLiggins Institute, University of Auckland, Auckland, New Zealand.
Cherie BlenkironHub for Extracellular Vesicles Investigations (HEVI), University of Auckland, Auckland, New Zealand.
Lawrence W ChamleyDepartment of Obstetrics and Gynaecology, School of Medicine, University of Auckland, Auckland, New Zealand.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trophoblasts are placenta-specific epithelial cells that play an essential role in conducting nutrient, gas, and waste exchange between the fetus and the mother. Primary culture of human trophoblasts from donated term placentae is an important tool to study placental functions. Currently, there is a lack of general consensus of the optimal culture conditions for maintaining term trophoblast cells in vitro. A key problem with culturing trophoblasts from term placentae is overgrowth of the trophoblasts by rapidly proliferating cellular contaminants. Recently we reported a system to culture trophoblasts from term placentae which differentiate into syncytiotrophoblast-like multinucleated cells that can be maintained in culture for at least 30 days with minimal contamination. This chapter details our optimized approach for long-term, contaminant-free in vitro culture of primary trophoblasts from term placentae.

Indexed as

PlacentaTrophoblastsConsensusDrug ContaminationEpithelial CellsFemaleHumansPregnancyCytotrophoblastHuman term placentaLong-term culturePrimary cultureSyncytiotrophoblastTrophoblast

Identifiers

PMID38019387

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.