Evidence map›Paper›PMID 38018989›Full record

ArticleMicrobiology spectrum2024

CRISPR-Cas9-mediated IncF plasmid curing in extraintestinal pathogenic

Liang Chen, Gisele Peirano, Kelly Yen, Bingjie Wang, Austin Terlecky, Rebekah DeVinney, Barry N Kreiswirth, Johann D D Pitout

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed, 1 pooled it
0.4field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 1 synthesis or guideline pooled it, 4 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Review
  4. Review
  5. The fitness connection of antibiotic resistance.Frontiers in microbiology · 2025
    Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 4 countries.

Liang ChenCenter for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.ORCID 0000-0001-5845-2235
Gisele PeiranoCummings School of Medicine, University of Calgary , Calgary, Alberta, Canada.
Kelly YenCenter for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.
Bingjie WangCenter for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.
Austin TerleckyCenter for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.
Rebekah DeVinneyCummings School of Medicine, University of Calgary , Calgary, Alberta, Canada.
Barry N KreiswirthCenter for Discovery and Innovation, Hackensack-Meridian Health , Nutley, New Jersey, USA.ORCID 0000-0002-0243-131X
Johann D D PitoutCummings School of Medicine, University of Calgary , Calgary, Alberta, Canada.
Hackensack Meridian Health · USUniversity of Calgary · CATongji University · CN

Funding

The molecular basis of the epidemic blaKPC gene KlebsiellaR01AI090155 · NIAID · UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL · PI KREISWIRTH, BARRY NEAL · 2011 to 2021
$7.2M
Identifying alcohol-related and built environment factors that can be modified to prevent pedestrian road traffic deathR01AA028552 · NIAAA · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI RUNDLE, ANDREW G · 2021 to 2024
$2.6M
NIAAA NIH HHS R01 AA028552NIAID NIH HHS R01 AI090155
6 · The paper itself

Abstract

importanceUnderstanding the role of IncF plasmids in the success of drug-resistant bacteria has far-reaching implications for tackling antibiotic resistance. The study's use of a novel CRISPR-Cas9-mediated plasmid-curing system provides a precision tool for dissecting the specific impact of IncF plasmids on ExPEC clones, especially high-risk, multidrug-resistant strains like ST131, ST1193, and ST410. The study offers a crucial stepping stone for future research into understanding how these plasmids influence more complex aspects of bacterial behavior, such as cell invasion and

Indexed as

Escherichia coli InfectionsExtraintestinal Pathogenic Escherichia coliAnti-Bacterial AgentsCRISPR-Cas SystemsHumansPlasmidsAnti-Bacterial AgentsCRISPRextraintestinal pathogenic Escherichia colipCasCureplasmid

Identifiers

PMID38018989
PMCPMC10783119
OpenAlexW4389136684

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.