Evidence map›Paper›PMID 38014311›Full record

ArticlebioRxiv : the preprint server for biology2023

A Method for Analyzing AFM Force Mapping Data Obtained from Soft Tissue Cryosections.

Cydney A Wong, Nina Sara Fraticelli Guzmán, A Thomas Read, Adam Hedberg-Buenz, Michael G Anderson, Andrew J Feola, Todd Sulchek, C Ross Ethier

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Cydney A WongWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA, USA.ORCID 0000-0001-9381-5847
Nina Sara Fraticelli GuzmánWoodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.ORCID 0000-0002-6148-0429
A Thomas ReadWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA, USA.
Adam Hedberg-BuenzDepartment of Molecular Physiology & Biophysics, University of Iowa, Iowa City, IA.
Michael G AndersonDepartment of Molecular Physiology & Biophysics, University of Iowa, Iowa City, IA.ORCID 0000-0001-5730-6105
Andrew J FeolaWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA, USA.ORCID 0000-0002-7914-989X
Todd SulchekWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA, USA.
C Ross EthierWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA, USA.ORCID 0000-0001-6110-3052
Georgia Institute of Technology · USUniversity of Iowa · US

Funding

P30-Core Grant for Vision Research Core CP30EY006360 · NEI · EMORY UNIVERSITY · PI GEISERT, ELDON E · 1986 to 2025
$15.3M
VIsion Science Training in AtlantaT32EY007092 · NEI · EMORY UNIVERSITY · PI Machelle T. Pardue · 1985 to 2026
$4.6M
Transcriptional and Biomechanical Analysis of Segmental Outflow in GlaucomaR01EY031710 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI ETHIER, C ROSS, GUPTA, DIVAKAR · 2020 to 2023
$2.4M
T32 CTEng (Cellular and Tissue Engineering) Training ProgramT32GM145735 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI Edward A. Botchwey, Andres J Garcia · 2022 to 2026
$2.3M
Impact of Menopause on the Aqueous Outflow PathwayR01EY030871 · NEI · GEORGIA INSTITUTE OF TECHNOLOGY · PI FEOLA, ANDREW J · 2020 to 2024
$1.6M
Mechanism of APBB2 contributions to glaucomaR21EY031598 · NEI · UNIVERSITY OF IOWA · PI ANDERSON, MICHAEL G, FINGERT, JOHN H · 2020 to 2021
$418k
The Effect of Estrogen Deficiencies on Vision Loss in GlaucomaIK2RX002342 · VA · VETERANS HEALTH ADMINISTRATION · PI FEOLA, ANDREW J · 2018 to 2023
–
NEI NIH HHS P30 EY006360NEI NIH HHS R01 EY030871NEI NIH HHS R01 EY031710NEI NIH HHS R21 EY031598NEI NIH HHS T32 EY007092NIGMS NIH HHS T32 GM145735RRD VA IK2 RX002342
6 · The paper itself

Abstract

Atomic force microscopy (AFM) is a valuable tool for assessing mechanical properties of biological samples, but interpretations of measurements on whole tissues can be difficult due to the tissue's highly heterogeneous nature. To overcome such difficulties and obtain more robust estimates of tissue mechanical properties, we describe an AFM force mapping and data analysis pipeline to characterize the mechanical properties of cryosectioned soft tissues. We assessed this approach on mouse optic nerve head and rat trabecular meshwork, cornea, and sclera. Our data show that the use of repeated measurements, outlier exclusion, and log-normal data transformation increases confidence in AFM mechanical measurements, and we propose that this methodology can be broadly applied to measuring soft tissue properties from cryosections.

Identifiers

PMID38014311
PMCPMC10680563
OpenAlexW4388648782

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.