Evidence map›Paper›PMID 38014270›Full record

ArticlebioRxiv : the preprint server for biology2023

Fungi of the order Mucorales express a "sealing-only" tRNA ligase.

Khondakar Sayef Ahammed, Ambro van Hoof

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Khondakar Sayef AhammedDepartment of Microbiology and Molecular Genetics. UT MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences. University of Texas Health Science Center at Houston.ORCID 0000-0001-9403-0937
Ambro van HoofDepartment of Microbiology and Molecular Genetics. UT MD Anderson Cancer Center UTHealth Houston Graduate School of Biomedical Sciences. University of Texas Health Science Center at Houston.ORCID 0000-0002-7800-9764
The University of Texas MD Anderson Cancer Center · US

Funding

RNAse functions in post-transcriptional gene regulationR35GM141710 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI VAN HOOF, AMBRO · 2021 to 2025
$2.4M
NIGMS NIH HHS R35 GM141710
6 · The paper itself

Abstract

Some eukaryotic pre-tRNAs contain an intron that is removed by a dedicated set of enzymes. Intron-containing pre-tRNAs are cleaved by tRNA splicing endonuclease (TSEN), followed by ligation of the two exons and release of the intron. Fungi use a "heal and seal" pathway that requires three distinct catalytic domains of the tRNA ligase enzyme, Trl1. In contrast, humans use a "direct ligation" pathway carried out by RTCB, an enzyme completely unrelated to Trl1. Because of these mechanistic differences, Trl1 has been proposed as a promising drug target for fungal infections. To validate Trl1 as a broad-spectrum drug target, we show that fungi from three different phyla contain Trl1 orthologs with all three domains. This includes the major invasive human fungal pathogens, and these proteins each can functionally replace yeast Trl1. In contrast, species from the order Mucorales, including the pathogens

Indexed as

antifungal targetTRL1tRNA splicing

Identifiers

PMID38014270
PMCPMC10680797
OpenAlexW4388749909

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.