Evidence map›Paper›PMID 38014113›Full record

ArticlebioRxiv : the preprint server for biology2023

A spatially-resolved transcriptional atlas of the murine dorsal pons at single-cell resolution.

Stefano Nardone, Roberto De Luca, Antonino Zito, Nataliya Klymko, Dimitris Nicoloutsopoulos, Oren Amsalem, Cory Brannigan, Jon M Resch, Christopher L Jacobs, Deepti Pant and 12 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 5 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

22 authors at 5 institutions in 2 countries.

Stefano NardoneDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Roberto De LucaDepartment of Neurology, Division of Sleep Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, 02215, USA.
Antonino ZitoDepartment of Twin Research & Genetic Epidemiology, King's College London, London, UK.ORCID 0000-0003-1931-984X
Nataliya KlymkoDivision of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave., Boston, MA 02215, USA.
Dimitris NicoloutsopoulosUCL Queen Square Institute of Neurology, University College London, London, UK.
Oren AmsalemDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Cory BranniganHEAVY.AI, 100 Montgomery St Fl 5, San Francisco, California, 94104, USA.
Jon M ReschDepartment of Neuroscience and Pharmacology, University of Iowa, Iowa City, IA, USA.
Christopher L JacobsDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Deepti PantDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Molly VereggeDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Harini SrinivasanDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Ryan M GrippoDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Zongfang YangDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Mark L ZeidelDivision of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave., Boston, MA 02215, USA.
Mark L AndermannDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Kenneth D HarrisUCL Queen Square Institute of Neurology, University College London, London, UK.
Linus T TsaiDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Elda ArrigoniDepartment of Neurology, Division of Sleep Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, 02215, USA.
Anne M J VerstegenDivision of Nephrology, Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School, 330 Brookline Ave., Boston, MA 02215, USA.
Clifford B SaperDepartment of Neurology, Division of Sleep Medicine, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, 02215, USA.
Bradford B LowellDepartment of Medicine, Division of Endocrinology, Diabetes and Metabolism, Beth Israel Deaconess Medical Center and Harvard Medical School, Boston, MA, USA.
Beth Israel Deaconess Medical Center · USBroad Institute · USNational Hospital for Neurology and Neurosurgery · GBKing's College London · GBUniversity of Iowa · US

Funding

Project 5: Novel strategies to prevent opioid-induced hypoventilation during sleep and identification of underlying mechanismsP01HL149630 · NHLBI · BETH ISRAEL DEACONESS MEDICAL CENTER · PI CLIFFORD B SAPER · 2020 to 2026
$22.3M
The routing of hunger/satiety information by the parabrachial nucleus (PBN)R01DK075632 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI BRADFORD B LOWELL · 2006 to 2026
$8.1M
Neuromodulatory mechanisms underlying vagus nerve stimulation therapy for Alzheimer's diseaseDP1AT010971 · NCCIH · BETH ISRAEL DEACONESS MEDICAL CENTER · PI ANDERMANN, MARK L · 2019 to 2023
$6.6M
Circuitry for Circadian RhythmsR01NS072337 · NINDS · BETH ISRAEL DEACONESS MEDICAL CENTER · PI SAPER, CLIFFORD B · 2010 to 2023
$4.8M
Mapping brainstem control of continenceR01DK113030 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI ZEIDEL, MARK L. · 2017 to 2021
$1.9M
Bladder fullness signaling and the neural control of continenceR01DK125708 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI VERSTEGEN, ANNE ("HANNEKE") · 2020 to 2024
$1.9M
Basal forebrain hypothalamic networks supporting wakefulnessR01NS091126 · NINDS · BETH ISRAEL DEACONESS MEDICAL CENTER · PI ARRIGONI, ELDA · 2015 to 2019
$1.9M
Central Regulation of Sodium Appetite via Synergistic Action of RAAS-sensitive NeuronsR00HL144923 · NHLBI · UNIVERSITY OF IOWA · PI RESCH, JON · 2021 to 2023
$747k
Single cell transcriptome profiling to define cell types in brain nuclei controlling bladder functionP20DK119789 · NIDDK · BETH ISRAEL DEACONESS MEDICAL CENTER · PI ZEIDEL, MARK L. · 2018 to 2019
$700k
SOCIAL INFLUENCES ON NEURAL PLASTICITYF32MH012343 · NIMH · UNIVERSITY OF WASHINGTON · PI WENNSTROM, KIRA L · 1999 to 2001
$46k
NCCIH NIH HHS DP1 AT010971NHLBI NIH HHS P01 HL149630NHLBI NIH HHS R00 HL144923NIDDK NIH HHS P20 DK119789NIDDK NIH HHS R01 DK075632NIDDK NIH HHS R01 DK113030NIDDK NIH HHS R01 DK125708NIMH NIH HHS F32 MH012343NINDS NIH HHS R01 NS072337NINDS NIH HHS R01 NS091126
6 · The paper itself

Abstract

The "dorsal pons", or "dorsal pontine tegmentum" (dPnTg), is part of the brainstem. It is a complex, densely packed region whose nuclei are involved in regulating many vital functions. Notable among them are the parabrachial nucleus, the Kölliker Fuse, the Barrington nucleus, the locus coeruleus, and the dorsal, laterodorsal, and ventral tegmental nuclei. In this study, we applied single-nucleus RNA-seq (snRNA-seq) to resolve neuronal subtypes based on their unique transcriptional profiles and then used multiplexed error robust fluorescence in situ hybridization (MERFISH) to map them spatially. We sampled ~1 million cells across the dPnTg and defined the spatial distribution of over 120 neuronal subtypes. Our analysis identified an unpredicted high transcriptional diversity in this region and pinpointed many neuronal subtypes' unique marker genes. We also demonstrated that many neuronal subtypes are transcriptionally similar between humans and mice, enhancing this study's translational value. Finally, we developed a freely accessible, GPU and CPU-powered dashboard (http://harvard.heavy.ai:6273/) that combines interactive visual analytics and hardware-accelerated SQL into a data science framework to allow the scientific community to query and gain insights into the data.

Identifiers

PMID38014113
PMCPMC10680649
OpenAlexW4386863038

What OpenQuestion holds

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LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.