ArticlePLoS genetics2023
Efficient sex separation by exploiting differential alternative splicing of a dominant marker in Aedes aegypti.
Article in PLoS genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 23 citations in OpenAlex.
- An OpIE2-DsRed marker disrupts female blood-feeding and shortens lifespan in the malaria vector Anopheles gambiae.Genetics · 2026Article
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Leveraging sex determination systems for genetic biocontrol of dipteran pests.Current opinion in insect science · 2026Review
- Advances in male sex separation for the support of mosquito control programs.Frontiers in insect science · 2026Review
- A male-drive female-sterile system for the self-limited control of the malaria mosquito Anopheles gambiae.Nature communications · 2025Article
- Mosquito sex determination: recent advances and applications.Current opinion in insect science · 2025Review
- Estimated cost and operational structure of pgSIT malaria vector control programs in selected West African countries.Scientific African · 2025Article
- CRISPR technologies for the control and study of malaria-transmitting anopheline mosquitoes.Parasites & vectors · 2025Review
- Differential elimination of marked sex chromosomes enables production of nontransgenic male mosquitoes in a single strain.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Genome Editing in the Yellow Fever Mosquito Aedes aegypti using CRISPR-Cas9.Journal of visualized experiments : JoVE · 2025Article
- Evaluation of ebony as a potential selectable marker for genetic sexing in Aedes aegypti.Parasites & vectors · 2025Article
- Evaluating the cost of malaria elimination by Anopheles gambiae precision guided SIT in the Upper River region, The Gambia.PLOS global public health · 2025Article
- Establishing a dominant early larval sex-selection strain in the Asian malaria vector Anopheles stephensi.Infectious diseases of poverty · 2024Article
- Eliminating malaria vectors with precision-guided sterile males.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- A Fluorescent Sex-Sorting Technique for Insects with the Demonstration inGEN biotechnology · 2024Article
- Next-generation genetic sexing strain establishment in the agricultural pest Ceratitis capitata.Scientific reports · 2023Article
- A fluorescent sex-sorting technique for insects with the demonstration inbioRxiv : the preprint server for biology · 2023Article
- Eliminating Malaria Vectors with Precision Guided Sterile Males.bioRxiv : the preprint server for biology · 2023Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Only female mosquitoes consume blood giving them the opportunity to transmit deadly human pathogens. Therefore, it is critical to remove females before conducting releases for genetic biocontrol interventions. Here we describe a robust sex-sorting approach termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter) that exploits sex-specific alternative splicing of an innocuous reporter to ensure exclusive dominant male-specific expression. Using SEPARATOR, we demonstrate reliable sex selection from early larval and pupal stages in Aedes aegypti, and use a Complex Object Parametric Analyzer and Sorter (COPAS) to demonstrate scalable high-throughput sex-selection of first instar larvae. Additionally, we use this approach to sequence the transcriptomes of early larval males and females and find several genes that are sex-specifically expressed. SEPARATOR can simplify mass production of males for release programs and is designed to be cross-species portable and should be instrumental for genetic biocontrol interventions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.