ArticleUltramicroscopy2024
Accurate magnification determination for cryoEM using gold.
Article in Ultramicroscopy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 11 citations in OpenAlex.
- Article
- Review
- WebCalEM: a browser-based tool for routine and accurate pixel-size calibration in cryo-EM.Acta crystallographica. Section F, Structural biology communications · 2026Article
- WebCalEM: a browser-based tool for routine and accurate pixel size calibration in cryo-EM.bioRxiv : the preprint server for biology · 2026Article
- The rise and fall of SARM1 base-exchange inhibitors.Communications chemistry · 2026Article
- Measurement of atomic scattering factors by cryoelectron microscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Leopard-EM: an extensible 2D template-matching package to accelerate in situ structural biology.Acta crystallographica. Section D, Structural biology · 2026Article
- Reducing the effects of radiation damage in cryo-EM using liquid helium temperatures.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A strategic approach for efficient cryo-EM grid optimization using design of experiments.Journal of structural biology · 2025Article
- Multi-species cryoEM calibration and workflow verification standard.Acta crystallographica. Section F, Structural biology communications · 2024Article
- Structure of Aquifex aeolicus lumazine synthase by cryo-electron microscopy to 1.42 Å resolution.IUCrJ · 2024Article
- Structure determination by cryoEM at 100 keV.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Determining the correct magnified pixel size of single-particle cryoEM micrographs is necessary to maximize resolution and enable accurate model building. Here we describe a simple and rapid procedure for determining the absolute magnification in an electron cryomicroscope to a precision of <0.5%. We show how to use the atomic lattice spacings of crystals of thin and readily available test specimens, such as gold, as an absolute reference to determine magnification for both room temperature and cryogenic imaging. We compare this method to other commonly used methods, and show that it provides comparable accuracy in spite of its simplicity. This magnification calibration method provides a definitive reference quantity for data analysis and processing, simplifies the combination of multiple datasets from different microscopes and detectors, and improves the accuracy with which the contrast transfer function of the microscope can be determined. We also provide an open source program, magCalEM, which can be used to accurately estimate the magnified pixel size of a cryoEM dataset ex post facto.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.