ArticleMethods in molecular biology (Clifton, N.J.)2024
Detection, Quantification, and Elimination of Wolbachia in Bed Bugs.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
4 citing papers in PubMed, 5 citations in OpenAlex.
- Counting cytoplasmic incompatibility factor mRNA using digital droplet PCR.Microbiology spectrum · 2026Article
- Wolbachia-supplemented B-vitamins are critical for blood digestion in the bed bug Cimex lectularius.Scientific reports · 2025Article
- Counting rareMicrobiology spectrum · 2025Article
- Prevalence of Heritable Symbionts in Parisian Bedbugs (Hemiptera: Cimicidae).Environmental microbiology reports · 2025Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Wolbachia is an obligatory nutritional symbiont of the common bed bug, Cimex lectularius, providing B-vitamins to its host. The biological significance of Wolbachia to bed bugs is investigated primarily by eliminating the symbiont with antibiotics, which is followed by confirmation with molecular assays. In this chapter, we describe a protocol for eliminating Wolbachia in bed bugs using the ansamycin antibiotic rifampicin (also known as rifampin) and three molecular methods to accurately detect and quantify the Wolbachia gene copies in bed bug samples. We describe the digital droplet PCR (ddPCR), a highly sensitive technique for absolute quantification of low abundance target genes, which has proven to be a valuable technique for confirmation of the elimination of Wolbachia.
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Registered trials
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