Evidence map›Paper›PMID 38004680›Full record

ArticleMicroorganisms2023

Cellulose Synthase in Atacama Cyanobacteria and Bioethanol Production from Their Exopolysaccharides.

Alexandra Galetović, Gabriel Peña, Nicole Fernández, Milton Urrutia, Nataly Flores, Benito Gómez-Silva, Jocelyne Di Ruggiero, Carolina Shene, Mariela Bustamante

Open access · goldAbstract read
In one paragraph

Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 2 countries.

Alexandra GaletovićLaboratorio de Bioquímica, Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Av. Universidad de Antofagasta 02800, Campus Coloso, Antofagasta 1271155, Chile.ORCID 0000-0001-9991-0781
Gabriel PeñaLaboratorio de Bioquímica, Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Av. Universidad de Antofagasta 02800, Campus Coloso, Antofagasta 1271155, Chile.
Nicole FernándezLaboratorio de Bioquímica, Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Av. Universidad de Antofagasta 02800, Campus Coloso, Antofagasta 1271155, Chile.
Milton UrrutiaCiencias Médicas, Facultad de Medicina y Odontología, Universidad de Antofagasta, Av. Argentina 2000, Antofagasta 1270001, Chile.
Nataly FloresCentre for Biotechnology and Bioengineering, CeBiB, Beauchef 851, North Building-7th Floor, Santiago 8370456, Chile.
Benito Gómez-SilvaLaboratorio de Bioquímica, Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Av. Universidad de Antofagasta 02800, Campus Coloso, Antofagasta 1271155, Chile.ORCID 0000-0002-7611-9943
Jocelyne Di RuggieroDepartment of Biology and Department of Earth and Planetary Sciences, Johns Hopkins University, Baltimore, MD 21218, USA.
Carolina SheneDepartment of Chemical Engineering and Center of Food Biotechnology and Bioseparations, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco 4811230, Chile.ORCID 0000-0003-4646-038X
Mariela BustamanteScientific and Technological Bioresource Nucleus, BIOREN, Universidad de La Frontera, Casilla 54-D, Temuco 5468901, Chile.
Universidad de Antofagasta · CLUniversidad de La Frontera · CLFraunhofer Chile Research Foundation · CLJohns Hopkins University · US

Funding

ANID- Millennium Science Initiative Program ICN2021_044Centre for Biotechnology and Bioengineering CeBiB FB-0001Financiamiento Asistente de Investigación, VRIIP, Universidad de Antofagasta ANT20992-CC_3346Fondo de Innovación para la Competitividad Regional -FIC-R 4603IANAS (Interamerican Network of Academies of Sciences) Fellowships Award. John Hopkins University,USA; IANAS Seed Grant National Academy of Science-USA IANASSemillero de Investigación, Universidad de Antofagasta 5305
6 · The paper itself

Abstract

Cyanobacteria produce exopolysaccharides (EPSs) as an adaptative mechanism against ultraviolet radiation and desiccation. Cellulose is present in the extracellular polymeric substance in some cyanobacteria genera and it has been proposed as a raw material for biofuel production. The goal of this work was to evaluate the cellulose presence in EPS of Atacama cyanobacteria strains and its use as an alternative and innovative biological source to produce bioethanol. The presence of cellulose was evaluated using techniques of molecular biology, bioinformatics, and electronic microscopy. The conserved motif D,D,D,35QXXRW, characteristic of processive β-glycosyltransferase in all cellulose-producing organisms, was identified in the genome of the LLA-10 strain. This is evidence that cellulose synthase in the LLA-10 strain is a functional enzyme. EPS from Atacama cyanobacteria was hydrolyzed by β-glucosidases (cellobiase and cellulase) and the released glucose was yeast-fermented to ethanol. Ethanol production reached 172.69 ± 0.02 mg ethanol/g EPS after 48 h of incubation. These results are the first step in the evaluation of EPS produced by native cyanobacteria isolated from northern Chile for future biotechnological applications such as the production of bioethanol.

Indexed as

bioethanolcellulosecellulose synthasecyanobacteriaexopolysaccharides

Identifiers

PMID38004680
PMCPMC10673042
OpenAlexW4388017852

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.