Evidence map›Paper›PMID 38004672›Full record

ArticleMicroorganisms2023

Sequencing of SARS-CoV-2 RNA Fragments in Wastewater Detects the Spread of New Variants during Major Events.

Damir Zhakparov, Yves Quirin, Yi Xiao, Nicole Battaglia, Michael Holzer, Martin Bühler, Walter Kistler, Doortje Engel, Jon Paulin Zumthor, Alexa Caduff and 1 more

Abstract read
In one paragraph

Article in Microorganisms, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Damir ZhakparovSwiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, 7265 Davos, Switzerland.ORCID 0000-0001-7175-0843
Yves QuirinCantonal Office for Nature and Environment, 7000 Chur, Switzerland.
Yi XiaoSwiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, 7265 Davos, Switzerland.
Nicole BattagliaCantonal Office for Food Security and Animal Health, 7000 Chur, Switzerland.
Michael HolzerCantonal Office for Nature and Environment, 7000 Chur, Switzerland.
Martin BühlerCantonal Office for Military and Civil Protection, 7000 Chur, Switzerland.
Walter KistlerHospital Davos, 7270 Davos, Switzerland.ORCID 0000-0002-9289-6953
Doortje EngelCantonal Office for Health, 7000 Chur, Switzerland.
Jon Paulin ZumthorCantonal Office for Food Security and Animal Health, 7000 Chur, Switzerland.
Alexa CaduffCantonal Office for Military and Civil Protection, 7000 Chur, Switzerland.ORCID 0000-0002-9008-6148
Katja BaerenfallerSwiss Institute of Allergy and Asthma Research (SIAF), University of Zurich, 7265 Davos, Switzerland.ORCID 0000-0002-1904-9440

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The sequencing of SARS-CoV-2 RNA in wastewater is an unbiased method to detect the spread of emerging variants and to track regional infection dynamics, which is especially useful in case of limited testing and clinical sequencing. To test how major international events influence the spread of new variants we have sequenced SARS-CoV-2 RNA in the wastewater samples of Davos, Landquart, Lostallo, and St. Moritz in the Swiss canton of Grisons in the time around the international sports competitions in Davos and St. Moritz in December 2021, and additionally in May 2022 and January 2023 in Davos and St. Moritz during the World Economic Forum (WEF) in Davos. The prevalence of the variants identified from the wastewater sequencing data showed that the Omicron variant BA.1 had spread in Davos and St. Moritz during the international sporting events hosted there in December 2021. This spread was associated with an increase in case numbers, while it was not observed in Landquart and Lostallo. Another instance of new variant spread occurred during the WEF in January 2023, when the Omicron variant BA.2.75 arrived in Davos but not in St. Moritz. We can therefore conclude that major international events promote the spread of new variants in the respective host region, which has important implications for the protective measures that should be taken.

Indexed as

amplicon sequencingCOVID-19 pandemicepidemiological surveillancerelative prevalenceSARS-CoV-2variant mappingwastewater

Identifiers

PMID38004672
PMCPMC10672834

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.