The Thermodynamic Fingerprints of Ultra-Tight Nanobody-Antigen Binding Probed via Two-Color Single-Molecule Coincidence Detection.

Benno Schedler, Olessya Yukhnovets, Lennart Lindner, Alida Meyer, Jörg Fitter

Abstract read

In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Macromolecular High-Affinity Binding Probed by Advanced Fluorescence Techniques.Chembiochem : a European journal of chemical biology · 2025
Review

4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Benno SchedlerAG Biophysik, I. Physikalisches Institut (IA), RWTH Aachen University, 52074 Aachen, Germany.ORCID 0009-0000-4050-2548

Olessya YukhnovetsAG Biophysik, I. Physikalisches Institut (IA), RWTH Aachen University, 52074 Aachen, Germany.

Lennart LindnerAG Biophysik, I. Physikalisches Institut (IA), RWTH Aachen University, 52074 Aachen, Germany.ORCID 0000-0001-9174-4352

Alida MeyerAG Biophysik, I. Physikalisches Institut (IA), RWTH Aachen University, 52074 Aachen, Germany.

Jörg FitterAG Biophysik, I. Physikalisches Institut (IA), RWTH Aachen University, 52074 Aachen, Germany.ORCID 0000-0002-4503-2079

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Life on the molecular scale is based on a versatile interplay of biomolecules, a feature that is relevant for the formation of macromolecular complexes. Fluorescence-based two-color coincidence detection is widely used to characterize molecular binding and was recently improved by a brightness-gated version which gives more accurate results. We developed and established protocols which make use of coincidence detection to quantify binding fractions between interaction partners labeled with fluorescence dyes of different colors. Since the applied technique is intrinsically related to single-molecule detection, the concentration of diffusing molecules for confocal detection is typically in the low picomolar regime. This makes the approach a powerful tool for determining bi-molecular binding affinities, in terms of

Indexed as

Reproducibility of ResultsDiffusionThermodynamicsantibody–antigen interactionbinding affinitybrightness-gated two-color coincidence detection (BTCCD)burst analysisconfocal fluorescence microscopynanobodiessingle-molecule fluorescence detectionthermodynamic parametersVan’t Hoff plot

Identifiers

PMID38003569

DOI10.3390/ijms242216379

PMCPMC10671529

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LicenceCC BY

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