Evidence map›Paper›PMID 38003179›Full record

ArticleAnimals : an open access journal from MDPI2023

Alterations in Gene Expression and the Fatty Acid Profile Impact but Do Not Compromise the In Vitro Maturation of Zebrafish (

Fernanda de Mello, Daniel Jaen Alonso, Natália Pires Vieira Morais de Faria, Victor Hugo Marques, Ethiene Fernandes de Oliveira, Paulo Henrique de Mello, Leandro César de Godoy, Renata Guimaraes Moreira

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animals : an open access journal from MDPI, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Fernanda de MelloDepartment of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil.ORCID 0000-0002-2998-6681
Daniel Jaen AlonsoDepartment of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil.
Natália Pires Vieira Morais de FariaDepartment of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil.
Victor Hugo MarquesDepartment of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil.
Ethiene Fernandes de OliveiraAquaculture Center, São Paulo State University "Julio de Mesquita Filho" (CAUNESP), Access Road Professor Paulo Donato Castellane, Vila Industrial District, Jaboticabal 14884-900, SP, Brazil.
Paulo Henrique de MelloBeacon Development, King Abdullah University of Science and Technology, 2713, Jeddah 23955, Saudi Arabia.
Leandro César de GodoyDepartment of Animal Science, Federal University of Rio Grande do Sul, 7712 Bento Gonçalves Avenue, Agronomia District, Porto Alegre 91540-000, RS, Brazil.ORCID 0000-0002-6236-9749
Renata Guimaraes MoreiraDepartment of Physiology, Bioscience Institute, University of Sao Paulo (IB/USP), 101 Matão Street, Travessa 14, Butantã District, São Paulo 05508-090, SP, Brazil.

Funding

National Council for Scientific and Technological Development 305493/2019-1National Council for Scientific and Technological Development 308784/2022-7São Paulo Research Foundation 2014/16320-7São Paulo Research Foundation 2017/21329-1São Paulo Research Foundation 2018/21422-4
6 · The paper itself

Abstract

The vitrification of ovarian follicles is a strategic tool that may contribute to advances in aquaculture and the conservation of many important species. Despite the difficulties inherent to the cryopreservation of oocytes, some successful protocols have been developed for different species, but little is known about the capacity of oocytes to develop after thawing. Therefore, the profiles of the reproductive pathway genes and fatty acid membrane composition during the initial stages of development were analyzed in fresh ovarian follicles and follicles after the vitrification process. There were differences in the expression of the hypothalamic-pituitary-gonad axis genes during the follicular development in the control group as well as in the vitrified group. Similarly, alterations in the composition of fatty acids were observed after vitrification. Despite this, many alterations were observed in the vitrified group; more than half of the stage III ovarian follicles were able to grow and mature in vitro. Therefore, the vitrification of ovarian follicles may impact them at molecular and membrane levels, but it does not compromise their capability for in vitro maturation, which indicates that the technique can be a strategic tool for aquaculture.

Indexed as

aquaculturefertility preservationfolliculogenesisgamete qualityoogenesisvitrification

Identifiers

PMID38003179
PMCPMC10668701

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.