Degradation Product of Sea Cucumber Polysaccharide by Dielectric Barrier Discharge Enhanced the Migration of Macrophage In Vitro.

Shiwen Cheng, Han Cai, Meng Yi, Liang Dong, Jingfeng Yang

Open access · goldAbstract read

In one paragraph

Article in Foods (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.2field-weighted citation impact, top 40% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Shiwen ChengSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.

Han CaiSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.

Meng YiSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.

Liang DongSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.

Jingfeng YangSchool of Food Science and Technology, Dalian Municipality Engineering Laboratory for Shellfish Polysaccharide, National Engineering Research Center of Seafood, Dalian Polytechnic University, Dalian 116034, China.

Dalian Polytechnic University · CN

Funding

National Natural Science Foundation of China 31771998

6 · The paper itself

Abstract

This study investigated the effect of dielectric barrier discharge (DBD) on sea cucumber polysaccharide (SP-2) and evaluated its anti-inflammatory properties. The SP-2 was depolymerized by applying an input voltage of 60~90 V for 3~9 min. The features of the products were examined using high-performance gel permeation chromatography, HPLC-PAD-MS, and the Fourier transform infrared (FTIR) spectrum. The anti-inflammatory properties of the product were investigated by measuring nitric oxide (NO) release, ROS accumulation, and cell migration using RAW264.7 cells (LPS-induced or not-induced). The results showed SP-2 depolymerized into homogeneous and controllable-size oligosaccharide products. The depolymerized ratio can reach 80%. The results of the measurement of reducing sugars indicate that SP-2 was cleaved from within the sugar chain. The SP-2 was deduced to have a monosaccharide sequence of GlcN-Man-Man-Man-Man-Man based on the digested fragment information. The depolymerization product restrained the release of NO and the accumulation of ROS. By testing the RAW264.7 cell scratch assay, it was found that it enhances the migration of immune cells. DBD degradation of SP-2 leads to homogeneous and controllable-size oligosaccharide products, and this technique can be used for polysaccharide structure analysis. The depolymerized product of SP-2 has an anti-inflammatory capability in vitro.

Indexed as

degradationdielectric barrier dischargemigrationpolysaccharideRAW264.7 cell

Identifiers

PMID38002137

DOI10.3390/foods12224079

PMCPMC10670309

OpenAlexW4388567509

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.