Evidence map›Paper›PMID 37998331›Full record

ArticleCells2023

Reporter Gene-Based qRT-PCR Assay for Rho-Dependent Termination In Vivo.

Monford Paul Abishek N, Heungjin Jeon, Xun Wang, Heon M Lim

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Disruption of Spore Coat Integrity inCurrent issues in molecular biology · 2025
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Monford Paul Abishek NDepartment of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea.ORCID 0000-0003-4553-1879
Heungjin JeonInfection Control Convergence Research Center, Chungnam National University College of Medicine, Daejeon 34134, Republic of Korea.ORCID 0000-0001-8704-3713
Xun WangNational Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Heon M LimDepartment of Biological Sciences, College of Biological Sciences and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea.ORCID 0000-0003-1711-2589

Funding

Chungnam National University 2020Fundamental Research Funds for the Central Universities 2662022SKYJ004National Key Research and Development Program of China 2022YFF1000700National Natural Science Foundation of China 31971339National Natural Science Foundation of China 32171422
6 · The paper itself

Abstract

In bacteria, the Rho protein mediates Rho-dependent termination (RDT) by identifying a non-specific cytosine-rich Rho utilization site on the newly synthesized RNA. As a result of RDT, downstream RNA transcription is reduced. Due to the bias in reverse transcription and PCR amplification, we could not identify the RDT site by directly measuring the amount of mRNA upstream and downstream of RDT sites. To overcome this difficulty, we employed a 77 bp reporter gene

Indexed as

RNARNA, Transfer, ArgBase SequenceGenes, ReporterReal-Time Polymerase Chain ReactionRNA, MessengerRNARNA, MessengerRNA, Transfer, ArgoperonqRT-PCRreporter geneRho-dependent termination

Identifiers

PMID37998331
PMCPMC10670590

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.