ArticleJournal of biological engineering2023
Discrimination between the effects of pulsed electrical stimulation and electrochemically conditioned medium on human osteoblasts.
Article in Journal of biological engineering, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 17 citations in OpenAlex.
- Bystander Effect Induced by Electrical Stimulation Promotes N2a Differentiation Through Interleukin-6.Biosensors · 2026Article
- In vitro electrical stimulation devices: practical framework for design, fabrication, and operation.Journal of biological engineering · 2026Article
- Parameter-resolved AC electrical stimulation links electrical system characteristics to early transcriptional responses in human stem cells.Frontiers in bioengineering and biotechnology · 2026Article
- Advantages of digital twin technology in orthopedic trauma Surgery - Exploring different clinical use cases.Scientific reports · 2025Article
- Advances in Electrical Materials for Bone and Cartilage Regeneration: Developments, Challenges, and Perspectives.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Tailoring Piezoelectric Nanogenerators and Microdevices for Cellular Excitation: Impact of Size and Morphology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Bioelectric stimulation outperforms brain derived neurotrophic factor in promoting neuronal maturation.Scientific reports · 2025Article
- Electrical stimulation for cartilage tissue engineering - A critical review from an engineer's perspective.Heliyon · 2024Review
- Electrical pulse stimulation parameters modulate N2a neuronal differentiation.Cell death discovery · 2024Article
- Effects of electrical stimulation with alternating fields on the osseointegration of titanium implants in the rabbit tibia - a pilot study.Frontiers in bioengineering and biotechnology · 2024Article
- Evaluating the electrical stimulation of bone cells based on an induced transmembrane potential model and intracellular calcium levels.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundElectrical stimulation is used for enhanced bone fracture healing. Electrochemical processes occur during the electrical stimulation at the electrodes and influence cellular reactions. Our approach aimed to distinguish between electrochemical and electric field effects on osteoblast-like MG-63 cells. We applied 20 Hz biphasic pulses via platinum electrodes for 2 h. The electrical stimulation of the cell culture medium and subsequent application to cells was compared to directly stimulated cells. The electric field distribution was predicted using a digital twin.
resultsCyclic voltammetry and electrochemical impedance spectroscopy revealed partial electrolysis at the electrodes, which was confirmed by increased concentrations of hydrogen peroxide in the medium. While both direct stimulation and AC-conditioned medium decreased cell adhesion and spreading, only the direct stimulation enhanced the intracellular calcium ions and reactive oxygen species.
conclusionThe electrochemical by-product hydrogen peroxide is not the main contributor to the cellular effects of electrical stimulation. However, undesired effects like decreased adhesion are mediated through electrochemical products in stimulated medium. Detailed characterisation and monitoring of the stimulation set up and electrochemical reactions are necessary to find safe electrical stimulation protocols.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.