Evidence map›Paper›PMID 37996909›Full record

ArticleJournal of neuroinflammation2023

Temporal changes in regulatory T cell subsets defined by the transcription factor Helios in stroke and their potential role in stroke-associated infection: a prospective case-control study.

Maria Lukasik, Magdalena Telec, Radoslaw Kazmierski, Izabela Wojtasz, Natalia Andrzejewska-Gorczyńska, Wojciech Kociemba, Grzegorz Dworacki, Wojciech P Kozubski, Magdalena Frydrychowicz

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroinflammation, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.9field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 7 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Maria LukasikDepartment of Neurology, Poznan University of Medical Sciences, Poznan, Poland. mlukasik@ump.edu.pl.
Magdalena TelecDepartment of Neurology, Poznan University of Medical Sciences, Poznan, Poland.
Radoslaw KazmierskiDepartment of Neurology, Collegium Medicum, University of Zielona Gora, Zielona Gora, Poland.
Izabela Wojtasz, Medicover, Poznan, Poland.
Natalia Andrzejewska-GorczyńskaDepartment of Neurology, Poznan University of Medical Sciences, Poznan, Poland.
Wojciech KociembaDepartment of Radiology, HCP Medical Center, Poznan, Poland.
Grzegorz DworackiDepartment of Immunology, Poznan University of Medical Sciences, Poznan, Poland.
Wojciech P KozubskiDepartment of Neurology, Poznan University of Medical Sciences, Poznan, Poland.
Magdalena FrydrychowiczDepartment of Immunology, Poznan University of Medical Sciences, Poznan, Poland.
Poznan University of Medical Sciences · PLUniversity of Zielona Góra · PL

Funding

National Science Centre, Poland 2014/15/B/NZ4/00736
6 · The paper itself

Abstract

backgroundRegulatory T cells (Tregs) are involved in the systemic immune response after ischemic stroke. However, their role remains unclear, and the effect appears to be both neuroprotective and detrimental. Treg suppressor function may result in immunodepression and promote stroke-associated infection (SAI). Thus we assume that the bidirectional effects of Tregs may be in part attributed to the intracellular transcription factor Helios. Tregs with Helios expression (H+ Tregs) constitute 70-90% of all Treg cells and more frequently than Helios-negative Tregs (H- Tregs) express molecules recognized as markers of Tregs with suppressor abilities. METHODS AND

resultsWe prospectively assessed the circulating Treg population with flow cytometry in 52 subjects on days 1, 3, 10 and 90 after ischemic stroke and we compared the results with those obtained in concurrent age-, sex- and vascular risk factor-matched controls. At all studied time points the percentage of H+ Tregs decreased in stroke subjects-D1: 69.1% p < 0.0001; D3: 62.5% (49.6-76.6), p < 0.0001; D10: 60.9% (56.5-72.9), p < 0.0001; D90: 79.2% (50.2-91.7), p = 0.014 vs. controls: 92.7% (81.9-97.0) and the percentage of H- Tregs increased accordingly. In patients with SAI the percentage of pro-suppressor H+ Tregs on post-stroke day 3 was higher than in those without infection (p = 0.03). After adjustment for confounders, the percentage of H+ Tregs on day 3 independently correlated with SAI [OR 1.29; CI 95%: 1.08-1.27); p = 0.02]. Although the percentage of H+ Tregs on day 3 correlated positively with NIHSS score on day 90 (rS = 0.62; p < 0.01) and the infarct volume at day 90 (rS = 0.58; p < 0.05), in regression analysis it was not an independent risk factor.

conclusionsOn the first day after stroke the proportion of H+ vs. H- Tregs changes in favor of pro-inflammatory H- Tregs, and this shift continues toward normalization when assessed on day 90. A higher percentage of pro-suppressive H+ Tregs on day 3 independently correlates with SAI and is associated positively with NIHSS score, but it does not independently affect the outcome and stroke area in the convalescent phase of stroke.

Indexed as

Ischemic StrokeT-Lymphocytes, RegulatoryCase-Control StudiesForkhead Transcription FactorsHumansIkaros Transcription FactorT-Lymphocyte SubsetsForkhead Transcription FactorsIkaros Transcription FactorIKZF2 protein, humanRegulatory T cellsStrokeStroke-associated infectionStroke immunologyTranscription factor Helios

Identifiers

PMID37996909
PMCPMC10666369
OpenAlexW4388931133

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.