ArticleNPJ Regenerative medicine2023
Epidermal-dermal coupled spheroids are important for tissue pattern regeneration in reconstituted skin explant cultures.
Article in NPJ Regenerative medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed, 15 citations in OpenAlex.
- Metabolic adaptation drives self-organization during skin organoid morphogenesis.Nature communications · 2026Article
- Hypoxia and metabolic adaptation drive self-organization in organoid morphogenesis and tissue regeneration.Burns & trauma · 2026Article
- HmmrResearch (Washington, D.C.) · 2026Article
- Deciphering the multifaceted role of double-stranded RNA sensor protein kinase R: pathophysiological function beyond the antiviral response.RNA biology · 2025Review
- Lifting regenerative barriers promotes epithelial cell fate plasticity supporting lineage conversion.Nature communications · 2025Article
- A Bioengineered Skin Organoid Platform for Modeling Human Skin Physiology and Cytotoxicity.Research square · 2025Article
- Regenerative Hair Pigmentation via Skin Organoids: Adaptive Patterning Mediated by Collagen VI and Semaphorin 3C.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Spatiotemporal Adaptations-Driven Dynamic Thra Activation Simulates a Skin Wound Healing Response.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Bioengineering of Microspheric Skin Organoids and Their Application in Drug Screening.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Hydrodynamically generated multilayer skin spheroids enable in vitro screening of biologically active ingredients and toxicity tests.Science advances · 2025Article
- Crosstalk between endothelial cells and dermal papilla entails hair regeneration and angiogenesis during aging.Journal of advanced research · 2025Article
- Functional regeneration strategies of hair follicles: advances and challenges.Stem cell research & therapy · 2025Review
- THSD4 promotes hair growth by facilitating dermal papilla and hair matrix interactions.Theranostics · 2025Article
- Endothelium-specific sensing of mechanical signals drives epidermal aging through coordinating retinoid metabolism.Theranostics · 2025Article
- Contact Cooling-Induced ELOVL4 Enhances Skin Wound Healing by Promoting the Inflammation-to-Proliferation Phase Transition.International journal of biological sciences · 2025Article
- Diversity of human skin three-dimensional organotypic cultures.Current opinion in genetics & development · 2024Review
- Energy competition remodels the metabolic glucose landscape of psoriatic epidermal cells.Theranostics · 2024Article
Corrections and comments
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Authors and funding
13 authors at 3 institutions in 4 countries.
Funding
Abstract
Tissue patterning is critical for the development and regeneration of organs. To advance the use of engineered reconstituted skin organs, we study cardinal features important for tissue patterning and hair regeneration. We find they spontaneously form spheroid configurations, with polarized epidermal cells coupled with dermal cells through a newly formed basement membrane. Functionally, the spheroid becomes competent morphogenetic units (CMU) that promote regeneration of tissue patterns. The emergence of new cell types and molecular interactions during CMU formation was analyzed using scRNA-sequencing. Surprisingly, in newborn skin explants, IFNr signaling can induce apical-basal polarity in epidermal cell aggregates. Dermal-Tgfb induces basement membrane formation. Meanwhile, VEGF signaling mediates dermal cell attachment to the epidermal cyst shell, thus forming a CMU. Adult mouse and human fetal scalp cells fail to form a CMU but can be restored by adding IFNr or VEGF to achieve hair regeneration. We find different multi-cellular configurations and molecular pathways are used to achieve morphogenetic competence in developing skin, wound-induced hair neogenesis, and reconstituted explant cultures. Thus, multiple paths can be used to achieve tissue patterning. These insights encourage more studies of "in vitro morphogenesis" which may provide novel strategies to enhance regeneration.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.