Evidence map›Paper›PMID 37996163›Full record

ArticleAnalytica chimica acta2023

Characterization of Gla proteoforms and non-Gla peptides of gamma carboxylated proteins: Application to quantification of prothrombin proteoforms in human plasma.

Dilip Kumar Singh, Abdul Basit, Allan E Rettie, Nathan Alade, Kenneth Thummel, Bhagwat Prasad

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytica chimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 49% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Dilip Kumar SinghDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, USA.
Abdul BasitDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, USA.
Allan E RettieDepartment of Medicinal Chemistry, University of Washington, Seattle, WA, USA.
Nathan AladeDepartment of Pharmaceutics, University of Washington, Seattle, WA, USA.
Kenneth ThummelDepartment of Pharmaceutics, University of Washington, Seattle, WA, USA.
Bhagwat PrasadDepartment of Pharmaceutical Sciences, Washington State University, Spokane, WA, USA. Electronic address: bhagwat.prasad@wsu.edu.
University of Washington · USWashington State University Spokane · US

Funding

Vitamin K gene network: Variant discovery and characterizationP01GM116691 · NIGMS · UNIVERSITY OF WASHINGTON · PI THUMMEL, KENNETH E. · 2016 to 2020
$7.6M
NIGMS NIH HHS P01 GM116691
6 · The paper itself

Abstract

Gamma (γ) carboxylation is an essential post-translational modification in vitamin K-dependent proteins (VKDPs), involved in maintaining critical biological homeostasis. Alterations in the abundance or activity of these proteins have pharmacological and pathological consequences. Importantly, low levels of fully γ-carboxylated clotting factors increase plasma des-γ-carboxy precursors resulting in little or no biological activity. Therefore, it is important to characterize the levels of γ-carboxylation that reflect the active state of these proteins. The conventional enzyme-linked immunosorbent assay for protein induced by vitamin K absence or antagonist II (PIVKA-II) quantification uses an antibody that is not applicable to distinguish different γ-carboxylation states. Liquid chromatography-mass spectrometry (LC-MS) approaches have been utilized to distinguish different γ-carboxylated proteoforms, however, these attempts were impeded by poor sensitivity due to spontaneous neutral loss of CO

Indexed as

ProthrombinVitamin KAdultHumansPeptidesProtein Processing, Post-TranslationalWarfarinPeptidesProthrombinVitamin KWarfarinGamma carboxylationGlobal proteomicsLC-MSProthrombinTargeted proteomicsVitamin K-dependent proteins

Identifiers

PMID37996163
PMCPMC10789187
OpenAlexW4388371158

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.