Evidence map›Paper›PMID 37995702›Full record

ArticleStem cell reports2023

Deriving early single-rosette brain organoids from human pluripotent stem cells.

Andrew M Tidball, Wei Niu, Qianyi Ma, Taylor N Takla, J Clayton Walker, Joshua L Margolis, Sandra P Mojica-Perez, Roksolana Sudyk, Lu Deng, Shannon J Moore and 7 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed, 2 pooled it
7.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 2 syntheses or guidelines pooled it, 46 citations in OpenAlex.

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  17. Advances in Cytotoxicity Testing: From In Vitro Assays to In Silico Models.International journal of molecular sciences · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

17 authors at 3 institutions in 1 country.

Andrew M TidballDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Wei NiuDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Qianyi MaDepartment of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, USA.
Taylor N TaklaDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
J Clayton WalkerDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Joshua L MargolisDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Sandra P Mojica-PerezDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Roksolana SudykDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Lu DengDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Shannon J MooreDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA.
Ravi ChopraDepartment of Neurology, Washington University School of Medicine, Saint Louis, MO, USA.
Vikram G ShakkottaiDepartment of Neurology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Geoffrey G MurphyDepartment of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA; Michigan Neuroscience Institute, University of Michigan Medical School, Ann Arbor, MI, USA.
Yukun YuanDepartment of Pharmacology, University of Michigan Medical School, Ann Arbor, MI, USA.
Lori L IsomDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA; Department of Molecular and Integrative Physiology, University of Michigan Medical School, Ann Arbor, MI, USA; Department of Pharmacology, University of Michigan Medical School, Ann Arbor, MI, USA.
Jun Z LiDepartment of Human Genetics, University of Michigan Medical School, Ann Arbor, MI, USA; Department of Computational Medicine and Bioinformatics, University of Michigan Medical School, Ann Arbor, MI, USA.
Jack M ParentDepartment of Neurology, University of Michigan Medical School, Ann Arbor, MI, USA; Michigan Neuroscience Institute, University of Michigan Medical School, Ann Arbor, MI, USA; VA Ann Arbor Healthcare System, Ann Arbor, MI, USA. Electronic address: parent@med.umich.edu.
University of Michigan · USThe University of Texas Southwestern Medical Center · USWashington University in St. Louis · US

Funding

Proteins to Cell SystemsU54NS117170 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI DANG, LOUIS TUONG CHINH · 2020 to 2024
$11.9M
Role of SCN1B in Inherited EpilepsyR37NS076752 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI ISOM, LORI L. · 2017 to 2023
$4.8M
Understanding Genetic Complexity in Spina BifidaR01HD111089 · NICHD · WEILL MEDICAL COLL OF CORNELL UNIV · PI RICHARD H. FINNELL, MARGARET ELIZABETH ROSS · 2023 to 2026
$2.8M
In vitro discovery and modeling of novel focal cortical dysplasia genesR21NS116250 · NINDS · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TIDBALL, ANDREW M · 2020 to 2020
$429k
Evaluating teratogenic risk of anti-seizure medications in single-rosette brain organoidsR21HD106580 · NICHD · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI TIDBALL, ANDREW M · 2021 to 2022
$215k
NICHD NIH HHS R01 HD111089NICHD NIH HHS R21 HD106580NINDS NIH HHS R21 NS116250NINDS NIH HHS R37 NS076752NINDS NIH HHS U54 NS117170
6 · The paper itself

Abstract

Brain organoid methods are complicated by multiple rosette structures and morphological variability. We have developed a human brain organoid technique that generates self-organizing, single-rosette cortical organoids (SOSR-COs) with reproducible size and structure at early timepoints. Rather than patterning a 3-dimensional embryoid body, we initiate brain organoid formation from a 2-dimensional monolayer of human pluripotent stem cells patterned with small molecules into neuroepithelium and differentiated to cells of the developing dorsal cerebral cortex. This approach recapitulates the 2D to 3D developmental transition from neural plate to neural tube. Most monolayer fragments form spheres with a single central lumen. Over time, the SOSR-COs develop appropriate progenitor and cortical laminar cell types as shown by immunocytochemistry and single-cell RNA sequencing. At early time points, this method demonstrates robust structural phenotypes after chemical teratogen exposure or when modeling a genetic neurodevelopmental disorder, and should prove useful for studies of human brain development and disease modeling.

Indexed as

Induced Pluripotent Stem CellsPluripotent Stem CellsBrainCell DifferentiationHumansOrganoidscortical spheroiddorsal forebrainepilepsyinduced pluripotent stem cellsmosaicismneural tube defectsneurodevelopmentneurulationPCDH19valproic acid

Identifiers

PMID37995702
PMCPMC10724074
OpenAlexW4388900432

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.