Evidence map›Paper›PMID 37994825›Full record

ArticleJournal of proteome research2024

Dilute to Enrich for Deeper Proteomics: A Yolk-Depleted Carrier for Limited Populations of Embryonic (Frog) Cells.

Leena R Pade, Camille Lombard-Banek, Jie Li, Peter Nemes

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of proteome research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Leena R PadeDepartment of Chemistry & Biochemistry, University of Maryland, College Park, Maryland 20742, United States.
Camille Lombard-BanekDepartment of Chemistry & Biochemistry, University of Maryland, College Park, Maryland 20742, United States.ORCID 0000-0003-0438-1042
Jie LiDepartment of Chemistry & Biochemistry, University of Maryland, College Park, Maryland 20742, United States.
Peter NemesDepartment of Chemistry & Biochemistry, University of Maryland, College Park, Maryland 20742, United States.ORCID 0000-0002-4704-4997
University of Maryland, College Park · US

Funding

Sub-Cellular Mass Spectrometry Discoveries: Metabolic Encoding of the Embryonic Body PlanR35GM124755 · NIGMS · UNIV OF MARYLAND, COLLEGE PARK · PI Peter Nemes · 2017 to 2026
$3.8M
NIGMS NIH HHS R35 GM124755
6 · The paper itself

Abstract

Abundant proteins challenge deep mass spectrometry (MS) analysis of the proteome. Yolk, the source of food in many developing vertebrate embryos, complicates chemical separation and interferes with detection. We report here a strategy that enhances bottom-up proteomics in yolk-laden specimens by diluting the interferences using a yolk-depleted carrier (YODEC) proteome via isobaric multiplexing quantification. This method was tested on embryos of the South African Clawed Frog (

Indexed as

ProteomeProteomicsAnimalsFlow CytometryMass SpectrometryXenopus laevisProteomedeyolkingmass spectrometryproteomicssensitivityXenopusyolk

Identifiers

PMID37994825
PMCPMC10872351
OpenAlexW4388941868

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.