Evidence map›Paper›PMID 37994727›Full record

ArticleNucleic acids research2024

JANUS, a spliceosome-associated protein, promotes miRNA biogenesis in Arabidopsis.

Mu Li, Huihui Yu, Bangjun Zhou, Lu Gan, Shengjun Li, Chi Zhang, Bin Yu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.0field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Non-canonical functions.Nature plants · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 2 countries.

Mu LiCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.ORCID 0000-0002-8442-7006
Huihui YuCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Bangjun ZhouCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Lu GanCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Shengjun LiKey Laboratory of Biofuels, Shandong Provincial Key Laboratory of Energy Genetics, Shandong Energy Institute, Qingdao New Energy Shangdong Laboratory, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266101, China.ORCID 0000-0003-0469-3222
Chi ZhangCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.
Bin YuCenter for Plant Science Innovation, University of Nebraska-Lincoln, Lincoln, NE 68588-0666, USA.ORCID 0000-0002-4763-177X
University of Nebraska–Lincoln · USQingdao Institute of Bioenergy and Bioprocess Technology · CN

Funding

Understand the function of the MOS4-associated complex in microRNA biogenesisR01GM127414 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI YU, BIN · 2018 to 2022
$1.6M
NIGMS NIH HHS R01 GM127414
6 · The paper itself

Abstract

MicroRNAs (miRNAs) are important regulators of genes expression. Their levels are precisely controlled through modulating the activity of the microprocesser complex (MC). Here, we report that JANUS, a homology of the conserved U2 snRNP assembly factor in yeast and human, is required for miRNA accumulation. JANUS associates with MC components Dicer-like 1 (DCL1) and SERRATE (SE) and directly binds the stem-loop of pri-miRNAs. In a hypomorphic janus mutant, the activity of DCL1, the numbers of MC, and the interaction of primary miRNA transcript (pri-miRNAs) with MC are reduced. These data suggest that JANUS promotes the assembly and activity of MC through its interaction with MC and/or pri-miRNAs. In addition, JANUS modulates the transcription of some pri-miRNAs as it binds the promoter of pri-miRNAs and facilitates Pol II occupancy of at their promoters. Moreover, global splicing defects are detected in janus. Taken together, our study reveals a novel role of a conserved splicing factor in miRNA biogenesis.

Indexed as

ArabidopsisArabidopsis ProteinsMicroRNAsGene Expression Regulation, PlantHumansRibonuclease IIIRNA Processing, Post-TranscriptionalRNA SplicingSpliceosomesArabidopsis ProteinsMicroRNAsRibonuclease III

Identifiers

PMID37994727
PMCPMC10783502
OpenAlexW4388939577

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.