ArticleAnalytical chemistry2023
Isolation of High-Molecular-Weight DNA for Long-Read Sequencing Using a High-Salt Gel Electroelution Trap.
Article in Analytical chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 20 citations in OpenAlex.
- Rapid Exonuclease-Based De Novo Sequencing Strategy for Long Oligonucleotides via Liquid-Phase Ion Trap Coupled With Mass Spectrometry.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Electroelution Into a Salt Trap: Reviving an Old-School Approach to DNA Purification.Small methods · 2026Article
- From sequencing to understanding: a grand challenge in genome-scale molecular and genetic analysis.Frontiers in plant science · 2026Article
- Genetic Diversity ofGenes · 2025Article
- Exploring Genetic Diversity and Inter-/Intraspecific Polymorphism inInternational journal of molecular sciences · 2025Article
- Developing an insert to protect large DNA molecules during cell lysis.BioTechniques · 2025Article
- Microfluidic purification of genomic DNA.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Polymorphic Loci of Adaptively Significant Genes Selection for Determining Nucleotide Polymorphism ofGenes · 2024Article
- An Improved Method and Device for Nucleic Acid Isolation Using a High-Salt Gel Electroelution Trap.Analytical chemistry · 2024Article
- Article
Corrections and comments
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Authors and funding
5 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long-read sequencing technologies require high-molecular-weight (HMW) DNA of sufficient purity and integrity, which can be difficult to obtain from complex biological samples. We propose a method for purifying HMW DNA that takes advantage of the fact that DNA's electrophoretic mobility decreases in a high-ionic-strength environment. The method begins with the separation of HMW DNA from various impurities by electrophoresis in an agarose gel-filled channel. After sufficient separation, a high-salt gel block is placed ahead of the DNA band of interest, leaving a gap between the separating gel and the high-salt gel that serves as a reservoir for sample collection. The DNA is then electroeluted from the separating gel into the reservoir, where its migration slows due to electrostatic shielding of the DNA's negative charge by excess counterions from the high-salt gel. As a result, the reservoir accumulates HMW DNA of high purity and integrity, which can be easily collected and used for long-read sequencing and other demanding applications without additional desalting. The method is simple and inexpensive, yields sequencing-grade HMW DNA even from difficult plant and soil samples, and has the potential for automation and scalability.
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Registered trials
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