ArticleJournal of experimental botany2024
A CRISPR/Cas9-based vector system enables the fast breeding of selection-marker-free canola with Rcr1-rendered clubroot resistance.
Article in Journal of experimental botany, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Effects of CRISPR technology on agricultural sustainability: global applications and turkish perspective.Transgenic research · 2026Review
- Synthetic Raphanobrassica Genome Reveals Functional and Evolutionary Insights Into Clubroot Resistance Genes on Chromosome R5.Plant biotechnology journal · 2026Article
- Beyond Resistance Genes: Silencing Susceptibility.International journal of molecular sciences · 2026Review
- Identification of candidate genes involved in root gall formation during early infection ofFrontiers in plant science · 2026Article
- A novel and efficient method for producing high-purity single protoplast-derived isolates ofFrontiers in microbiology · 2026Article
- Identification and characterization ofHorticulture research · 2025Article
- Identification of Rcr12, a single dominant clubroot resistance gene near Rcr6 on chromosome B3 of Brassica nigra.BMC plant biology · 2025Article
- Clubroot resistant in cruciferous crops: recent advances in genes and QTLs identification and utilization.Horticulture research · 2025Article
- Applications of CRISPR/Cas tools in improving stress tolerance inFrontiers in plant science · 2025Review
- Application of a multiplex CRISPR/Cas9 strategy for elimination of selection markers from transgenic plants.Frontiers in genome editing · 2025Article
- Genetic improvement of clubroot resistance in the recessive genic male sterile line YA001 inFrontiers in plant science · 2025Article
- Comprehensive review ofFrontiers in microbiology · 2025Review
- CRISPR mutant rapid identification inFrontiers in plant science · 2025Article
- Identification of three novel QTL for resistance to highly aggressive Canadian strains ofFrontiers in plant science · 2025Article
- Use of CRISPR Technology in Gene Editing for Tolerance to Biotic Factors in Plants: A Systematic Review.Current issues in molecular biology · 2024Review
- Resynthesizing Brassica napus with race specific resistance genes and race non-specific QTLs to multiple races of Plasmodiophora brassicae.Scientific reports · 2024Article
- Resilience of Canola toPlants (Basel, Switzerland) · 2024Article
- Identification of genetic loci and candidate genes regulating photosynthesis and leaf morphology through genome-wide association study inFrontiers in plant science · 2024Article
- Lignin accumulation in cell wall plays a role in clubroot resistance.Frontiers in plant science · 2024Article
- Comparative transcriptome analysis of canola carrying a single vs stacked resistance genes against clubroot.Frontiers in plant science · 2024Article
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3 authors.
Funding
Abstract
Breeding for disease resistance in major crops is of crucial importance for global food security and sustainability. However, common biotechnologies such as traditional transgenesis or genome editing do not provide an ideal solution, whereas transgenic crops free of selection markers such as cisgenic/intragenic crops might be suitable. In this study, after cloning and functional verification of the Rcr1 gene for resistance to clubroot (Plasmodiophora brassicae), we confirmed that the genes Rcr1, Rcr2, Rcr4, and CRa from Brassica rapa crops and the resistance gene from B. napus oilseed rape cv. 'Mendel' on chromosome A03 were identical in their coding regions. We also determined that Rcr1 has a wide distribution in Brassica breeding materials and renders potent resistance against multiple representative clubroot strains in Canada. We then modified a CRISPR/Cas9-based cisgenic vector system and found that it enabled the fast breeding of selection-marker-free transgenic crops with add-on traits, with selection-marker-free canola (B. napus) germplasms with Rcr1-rendered stable resistance to clubroot disease being successfully developed within 2 years. In the B. napus background, the intragenic vector system was able to remove unwanted residue sequences from the final product with high editing efficiency, and off-target mutations were not detected. Our study demonstrates the potential of applying this breeding strategy to other crops that can be transformed by Agrobacterium. Following the streamlined working procedure, intragenic germplasms can be developed within two generations, which could significantly reduce the breeding time and labor compared to traditional introgression whilst still achieving comparable or even better breeding results.
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