ArticleCell reports methods2023
Third-generation rabies viral vectors allow nontoxic retrograde targeting of projection neurons with greatly increased efficiency.
Article in Cell reports methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 16 citations in OpenAlex.
- POINTseq: Cell-type-specific barcoding reveals single-cell projection architecture of the mouse dopaminergic system.Neuron · 2026Article
- Ribosome remodeling drives translation adaptation during viral infection and cellular stress.Molecular cell · 2026Article
- An applicable and efficient retrograde monosynaptic circuit mapping tool for larval zebrafish.eLife · 2026Article
- Transsynaptic tracing techniques to interrogate neuronal connectivity of glioblastomas.Nature protocols · 2026Review
- Cell type-specific barcoding reveals the single-neuron projectional architecture of the mouse midbrain dopaminergic system.bioRxiv : the preprint server for biology · 2026Article
- Large-scale mapping of the MCH network in ALS mice reveals the vulnerability of dopaminergic and GABAergic neurons in zona incerta.Acta neuropathologica communications · 2026Article
- Circuit-specific gene editing for precision modulation of neuronal activity with CRISPR-rabies virus.bioRxiv : the preprint server for biology · 2025Article
- Hippocampal neural circuit mechanisms in Alzheimer's disease revealed by viral-genetic circuit mapping.Neurobiology of disease · 2025Review
- Programmable self-replicating JEV nanotherapeutics redefine RNA delivery in ALS.Communications biology · 2025Review
- A molecularly defined mPFC-BLA circuit specifically regulates social novelty preference.Science advances · 2025Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA and in situ sequencing.eLife · 2024Article
- Long-term labeling and imaging of synaptically connected neuronal networks in vivo using double-deletion-mutant rabies viruses.Nature neuroscience · 2024Article
- Third-generation rabies viral vectors have low toxicity and improved efficiency as retrograde labeling tools.Cell reports methods · 2023Article
- Rabies virus-based barcoded neuroanatomy resolved by single-cell RNA andbioRxiv : the preprint server for biology · 2023Article
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10 authors at 2 institutions in 1 country.
Funding
Abstract
Rabies viral vectors have become important components of the systems neuroscience toolkit, allowing both direct retrograde targeting of projection neurons and monosynaptic tracing of inputs to defined postsynaptic populations, but the rapid cytotoxicity of first-generation (ΔG) vectors limits their use to short-term experiments. We recently introduced second-generation, double-deletion-mutant (ΔGL) rabies viral vectors, showing that they efficiently retrogradely infect projection neurons and express recombinases effectively but with little to no detectable toxicity; more recently, we have shown that ΔGL viruses can be used for monosynaptic tracing with far lower cytotoxicity than the first-generation system. Here, we introduce third-generation (ΔL) rabies viral vectors, which appear to be as nontoxic as second-generation ones but have the major advantage of growing to much higher titers, resulting in significantly increased numbers of retrogradely labeled neurons in vivo.
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