Evidence map›Paper›PMID 37988619›Full record

ArticleACS synthetic biology2023

Synthetic Biology Toolbox for Nitrogen-Fixing Soil Microbes.

Maya Venkataraman, Audrey Yñigez-Gutierrez, Valentina Infante, April MacIntyre, Paulo Ivan Fernandes-Júnior, Jean-Michel Ané, Brian Pfleger

Abstract read
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

  1. Optimized cumate toolkit for tunable protein expression duringApplied and environmental microbiology · 2026
    Article
  2. Article
  3. Developing improved inoculants through genome editing of diazotrophic bacteria for cereals: Is this the right time for Brazil?Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
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  15. Development of modular expression across phylogenetically distinct diazotrophs.Journal of industrial microbiology & biotechnology · 2024
    Article
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Maya VenkataramanDepartment of Chemical and Biological Engineering, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.
Audrey Yñigez-GutierrezDepartment of Chemical and Biological Engineering, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.
Valentina InfanteDepartment of Bacteriology, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.
April MacIntyreDepartment of Bacteriology, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.ORCID 0000-0002-1867-6661
Paulo Ivan Fernandes-JúniorDepartment of Bacteriology, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.
Jean-Michel AnéDepartment of Bacteriology, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.
Brian PflegerDepartment of Chemical and Biological Engineering, University of Wisconsin─Madison, Madison, Wisconsin 53706, United States.ORCID 0000-0002-9232-9959

Funding

Institutional Training in the Genomic SciencesT32HG002760 · NHGRI · UNIVERSITY OF WISCONSIN-MADISON · PI Qiongshi Lu · 2003 to 2026
$17.7M
Biotechnology Training ProgramT32GM135066 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI SCOTT M. COYLE, BRIAN G FOX · 2020 to 2026
$7.0M
NHGRI NIH HHS T32 HG002760NIGMS NIH HHS T32 GM135066
6 · The paper itself

Abstract

The soil environment adjacent to plant roots, termed the rhizosphere, is home to a wide variety of microorganisms that can significantly affect the physiology of nearby plants. Microbes in the rhizosphere can provide nutrients, secrete signaling compounds, and inhibit pathogens. These processes could be manipulated with synthetic biology to enhance the agricultural performance of crops grown for food, energy, or environmental remediation, if methods can be implemented in these nonmodel microbes. A common first step for domesticating nonmodel organisms is the development of a set of genetic engineering tools, termed a synthetic biology toolbox. A toolbox comprises transformation protocols, replicating vectors, genome engineering (e.g., CRISPR/Cas9), constitutive and inducible promoter systems, and other gene expression control elements. This work validated synthetic biology toolboxes in three nitrogen-fixing soil bacteria:

Indexed as

NitrogenSoilCRISPR-Cas SystemsGenetic EngineeringPlasmidsSynthetic BiologyNitrogenSoilCas9CRISPRdiazotrophsgenome editingnitrogenasesynthetic biology toolbox

Identifiers

PMID37988619
PMCPMC10754042

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.